细胞因子、粘附分子和毒性蛋白在鼻息肉中的表达及意义

【作者】 康厚墉；

【导师】 洪苏玲；

【作者基本信息】 重庆医科大学 ， 耳鼻咽喉科学， 2004， 硕士

【摘要】 目的：探讨鼻息肉组织中嗜酸性粒细胞阳离子蛋白（ECP）、粘附分子E-选择素（E-selectin）和前炎症细胞因子粒细胞-巨噬细胞集落刺激因子（GM-CSF）水平及其在鼻息肉病理机理中的相关性。方法：采用酶联免疫吸附测定（enzyme linked immunosoent assay，ELISA）检测60例鼻息肉组织匀浆中ECP、E-selectin和GM-CSF水平，以12例鼻中隔手术患者之代偿肥大的下鼻甲后端粘膜作为对照。结果：鼻息肉组ECP浓度为293.72±56.87μg/L，对照组69.17±14.75μg/L；鼻息肉组E-selectin浓度为42.58±13.52μg/L，对照组为11.35±3.17μg/L；鼻息肉组GM-CSF浓度为83.75±38.58ng/L，对照组为9.87±2.22ng/L。鼻息肉组织匀浆中ECP、E-selectin和GM-CSF水平均明显高于对照组，差异极显著（P﹤0.001）。三种分子在鼻息肉中的表达相互间呈显著正相关。结论：ECP、E-selectin和GM-CSF相互协同作用，通过参与嗜酸性粒细胞等炎症细胞的浸润，活化，增殖，从而在鼻息肉的形成过程中起重要作用。更多还原

【Abstract】 Objective: To study significance of Eosinophil cationic protein (ECP), E-selectin and granulocyte-macrophage colony stimulation factor (GM-CSF) in nasal polyps.Methods: The ECP, E-selectin and GM-CSF were measured by enzyme linked immunosorbent assay (ELISA) in group of 60 cases and 12 control cases from normal inferior turbinates respectively. Results: The level of ECP in nasal polyps group was 293.72±56.87μg/L, while in control groups was 69.17±14.75μg/L; The level of E-selectin was 42.58±13.52μg/L，in control group was 11.35±3.17μg/L, The level of GM-CSF was 83.75±38.58ng/L, in control <WP=11>group was 9.87±2.22ng/L. The level of ECP, GM-CSF and E-selectin in nasal polyps groups was significantly higer than those of controls(P<0.001). And the strong positive correlations were observed among the three participants. Conclusion: This study demonstrate that the secretion of ECP, E-selectin and GM-CSF increase significantly in nasal polyps. They may involve the occurrence of nasal polyps by activiting on eosinophils.更多还原