【作者】 崔介君；

【导师】 孙培龙； 马新；

【作者基本信息】 浙江工业大学 ， 生物化工， 2004， 硕士

【摘要】 花生壳资源丰富，其黄酮成分具有多种重要的生理功能，在医药、化妆品、保健食品领域具有广阔的应用前景。本文建立了花生壳黄酮的高效提取纯化工艺，及其分析检测手段。此外，还对花生壳黄酮的抗氧化作用和抑菌活性进行了测定。本实验结果摘要如下： 1．黄酮提取的工艺参数：原料提取前经微波预处理15min～20min、并用石油醚脱脂1h，提取溶剂80％乙醇、温度50℃、时间2.5h、固液比=1：25(g／ml)、搅拌速度500r／min、颗粒粒度40目、pH=8.5； 2．AB-8为较佳黄酮吸附树脂，最佳工艺条件：4BV 75％乙醇洗脱、pH=6、L=250mg／ml、吸附速度U=3BV／h，解吸速度u=1BV／h，10BV15％乙醇洗涤； 3．应用上述工艺能得到黄酮含量在60％以上的产品； 4．提取物的添加浓度和抗氧化性之间呈明显的剂量-效应关系。提取物清除自由基的活性与其所含黄酮成分和结构存在一定的关系； 5．黄酮对金黄色葡萄球菌具有很强的抑制和杀灭作用，精制黄酮和纯化组分的作用明显优于粗提黄酮。更多还原

【Abstract】 Peanut hull is very abundant, the flavonoids of which are provided with many reported physiological function and applied in the fields of medicine, cosmetic and health food. The thesis has developed a highly efficient extraction and purification process of peanut hull flavonoids, including the analysis and determination of flavonoids, optimization of extraction and purification conditions, furthermore antioxidation and antibacterial activity of the peanut hull flavonoids are analyzed. The technology parameters and the results of functionality based on experiments are as the following:1.The optimum extracting technology parameters: raw materials treated with microwave for 15~20min and degreased by ether for 1 hour. 80% alcohol (v/v), T=50℃,t=2.5h, solid-liquid =1:25 (g/ml), mixing rate=500r/min, granularity=40mu, pH=8.5;2. AB-8 is a better resin compared with the others. The optimum condition: 4BV 75% alcohol for elution, pH=6, L=250mg/ml, adsorption U=3BV/h, desorption u=lBV/h ,10BV 15% alcohol for abstersion;3. Under the optimum condition of above, the content of fiavonoids in peanut hull eaxtraction obtained is over 60%.4. It has obviously showed the relationship of dosage-domino offect between the additive concentration and antioxidant ability of fiavonoids. There was a certain relationship between the activity of scavenging free radicals and the component and structure of fiavonoids;5. The fiavonoids can strongly inhibit and sterilize Staphylococcus aureus, the germicidal effect of the purified PHE and the purified fraction is obviously better than that of the PHE without purification.更多还原