【作者】 李映笙；

【导师】 陆祖康； 倪旭翔；

【作者基本信息】 浙江大学 ， 光学工程， 2004， 硕士

【摘要】 近10多年来，国内生物芯片技术的发展备受瞩目，在生命科学研究、疾病诊断、新药开发、生物武器、司法鉴定、食品卫生监督等等领域已越来越被广泛运用。然而在生物芯片技术中的重要组成部分之一——生物芯片的检测设备却几乎一直依赖国外的产品，其垄断性的昂贵价格，加上复杂的专业分析操作，对我们生物芯片的应用推广造成了许多障碍。因此，开发具有自主知识产权的检测扫描仪具有深远的意义。 本文提出了一种新颖的激光共焦生物芯片扫描仪。仪器基于激光共焦显微镜的理论，采用振镜和远心线性成像物镜实现X向扫描；精密导轨和步进电机实现Y向扫描的检测仪，经验证，扫描仪具有快速、操作简单、检测芯片能力良好等特征，有望弥补市场的空缺。 在本论文中，首先介绍了生物芯片技术的内涵，对检测系统的结构进行了比较分析，提出了检测仪的结构特点和存在的问题的解决方案。重点给出了检测系统研制中，信号的采集与传输的硬件和软件实现，实现了良好的无损数据采集和实时图像重建；然后通过采集成像实验，对扫描仪的性能参数即分辨率和灵敏度进行扫描分析，对系统结构加以调整和改进，最终达到了5um分辨率和接近1荧光分子／um~2的灵敏度指标。最后给出了深圳益生堂公司提供的丙肝病毒抗体蛋白芯片HCV芯片的检测实验结果。更多还原

【Abstract】 In the present ten years, domestic biochip technique has been developed enormously, and applied to many fields, such as life science research diagnosis, new drug development, bio-weapon war, judicial evaluation, foods and environment supervise etc. However, the biochip analyzer, which is one important part of biochip technique, is almost all depended on foreign ones. And for the two factors of high price because of monopoly and complicated use method because of adapting to research field, it brings many obstruction to the application and spread of biochip technique in our country. So it is a great significance to research and develop a biochip analyzer that own ourselves intellectual copyright and/or patent.A novel laser confocal biochip scanner was given in this paper. It was based on the principle of laser confocal microscope. And the two-dimensional scanning configuration was adopted by the optical scanner and the telecentric linear imaging objective lens of large numerical aperture to realize X-direction’s scanning, and the conventional mechanical method using linear driver and linear guide track to Y-direction’s. The experiment results indicate that the device can run smoothly and rapidly, be operated easily and detect fluorescence effectively.The biochip technique and the detection methods were introduced detailedly firstly in the paper, then the characteristic of the scanner researched and the problem and resolution of it were put forward. Mainly the signal processing about the realization of signal data acquisition and real-time imaging, the hardware setting and software programming were presented. Next, on the basis of lots of experiments the performance parameters about the resolution and sensitivity of the scanner were discussed. And by adjusting and modifying the system configuration, its resolution reached 5 micron and sensitivity about one fluorescent molecule per square micron.. In the end the experiment results of the biochip which was given by Shenzhen Yishengtang Biological Products Co,.Ltd were interpreted.更多还原