【作者】 唐斌；

【导师】 徐世清；

【作者基本信息】 苏州大学 ， 特种经济动物饲养， 2003， 硕士

【摘要】 依靠形态特征鉴定家蚕品种与纯度，易受环境影响，检验准确性差。本文应用DNA分子标记技术，利用RAPD特征和SCAR标记鉴定江苏省主要蚕品种及其纯度。结果找到了苏5、苏6，苏菊、明虎，苏镇、春光等品种的RAPD特征DNA片段，进一步将苏5、苏6，苏菊、明虎两组RAPD标记转换成特异性更强的SCAR标记，可以准确地用于苏5、苏6和苏菊、明虎品种及其后代的纯度鉴定。 核糖体失活蛋白(RIP)是生物体内存在的一类抑制真核生物核糖体蛋白质合成的蛋白质，可作为免疫毒素的毒弹头等，应用于治疗癌症和AIDS等多种疾病。本文对香樟Ⅱ型核糖体失活蛋白Cinnamomin进行了大规模的抽提和纯化。并将纯化的Cinnamomin蛋白与大鼠肝细胞80S核糖体作用，鉴定出其存在RNAN—糖苷酶的活性，确认其为核糖体失活蛋白。更多还原

【Abstract】 The method of identifying silkworm variety and purity with morphological characters is easily affected by environment and the accuracy is poor.In this paper, the DNA polymorphism bands have been amplified with RAPD among Jiangsu main silkworm races, Su5, Su6, Suju, Minghu, Suzhen, and Chunguang. Then respectively convert specific bands of Su5, Su6, Suju and Minghu into stable SCAR markers (Sequenced characterized amplified region).These SCAR markers can be used to determine variety of Su5, Su6, Suju and Minghu and the purity of their Fl hybrid.Ribosome-inactivating proteins (RIPs) are a group of cytoxin which chemically act on eukaryotic ribosome rRNA and inhibit protein biosynthesis. Increasing interests concentrate in this field due to the potential use of RIPs for treatment of several important human diseases such as cancer and AIDS. Here, a new method for large-scale preparation of Cinnamomin, a RIP from Cinnamomum camphora was established. In this paper the activity of RNA N-glycosidas of the purified Cinnamomin was identified. Rat liver 80S ribosomes were incubated with Cinnamomin. The purified Cinnamomin is a kind of RIP.更多还原