【作者】 吴银艳；

【导师】 许笃聪；

【作者基本信息】 福建中医学院 ， 中西医结合临床， 2003， 硕士

【摘要】 目的 通过动物实验观察补气活血化痰汤抗兔动脉粥样硬化斑块形成的作用及其对血浆内皮素—1(ET—1)和一氧化氮(NO)及血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL—C)、低密度脂蛋白胆固醇(LDL—C)、载脂蛋白A—1(ApoA—1)、载脂蛋白B(ApoB)水平的影响。 方法 采用免疫损伤加高胆固醇饮食方法建立新西兰大耳兔动脉粥样硬化模型，设立硝苯吡啶对照，观察补气活血化痰汤对动脉粥样硬化模型兔主动脉、冠状动脉粥样硬化病变及血脂、血浆NO、ET—1水平的影响。光镜下观察模型兔主动脉、冠状动脉的病变程度，酶法测定血脂、血浆NO水平，放射免疫法测定血浆ET—1含量。 结果 1．免疫损伤合并高胆固醇喂饲可诱发模型兔主动脉AS病变产生。 2．补气活血化痰汤能使模型兔主动脉AS病变减轻(P＜0.05)。 3．补气活血化痰汤能使ApoA—1、HDL—C、NO水平上升(P＜0.05、P＜0.01)，能使LDL—C、TC、ApoB、ET-1水平下降(P＜0.05、P＜0.01)。 结论 中药补气活血化痰汤具有较好的抗动脉粥样硬化作用，这可能与其能够有效地调节NO／ET-1平衡失调及血脂水平有关。更多还原

【Abstract】 Objective:To investigate the antiatherogenetic activity of Chinese medicine Buqihuoxuehuatan decoction in male rabbit fed an atherogenic diet and the effect of the total cholesterol, triglycerides, low-density lipoprotein-cholesterol apolipoprotein B high- density lipoprotein- cholesterol and apolipoprotein Al level in serum, and investigate the nitric oxide and endothlin-1 level in plasma.Materials and Methods:An atherosclerotic rabbit model was established by feeding high cholesterol diet supplemented by bovine serum albumin injection bolus. The rabbits were randomly divided into three groups: the model group, Buqihuoxuehuatan Decoction treated group and nifedipine treated group. After fed for twelve weeks, all animals were killed to take blood sample through heart to measure , and to take aortic to determine the percentage of aortic lesions covering the intimal surface and to observe structure with light microscope ,and coronary arteria sample to observe structure with light microscope.Results:1. Feeding high cholesterol diet supplemented by bovine serum albumin injection bolus can establish an atherosclerotic rabbit model.2. Buqihuoxuehuatan Decoction can decrease the aortic atherosclerosis (P < 0.05 ) .3. Buqihuoxuehuatan Decoction can increase the serum high-density lipoprotein-cholesterol apolipoprotein A1 and plasma nitric oxide, and decrease the serum total cholesterol, low-density lipoprotein-cholesterol, apolipoprotein B and the plasma endothlin-1 (P<0.05, P<0.01) .Conclusion:Buqihuoxuehuatan Decoction had antiatherogenic effects and its mechanism might associate with modifying the serum lipid , preserving endothelial function and maintaining the balance of NO and ET-1.更多还原