【作者】 廖文榕；

【导师】 陈国南； 吴晓苹；

【作者基本信息】 福州大学 ， 分析化学， 2003， 硕士

【摘要】 根据2000年公布的世界卫生报告，1千7百万人死于心血管疾病，即全球每3个死亡人中就有1个死于心血管疾病。心血管疾病成了全球卫生保健和卫生资源的巨大负担。治疗心血管疾病的药物主要有强心药、抗心律失常药、抗高血压药、抗心绞痛药、降脂药等。为了充分发挥各种药物的治疗潜力，保证安全合理用药，应建立这些药物灵敏度高、特异性强的快速检测方法。本文以盐酸胺碘酮、苯溴马隆、盐酸普鲁卡因胺、盐酸普鲁卡因等四种与治疗心血管病疾病有关的药物为研究对象，做了以下几方面的工作：在论文的第二章及第三章中，我们采用了循环伏安法（CV），线性扫描伏安法（LSV），计时库仑以及恒电位库仑电解法等现代电化学技术详细研究了苯并呋喃的衍生物：盐酸胺碘酮及苯溴马隆在玻碳电极(GCE)上的电化学氧化机理。同时建立了一个简单、快速、灵敏的胺碘酮微分脉冲伏安（DPV）检测体系；为苯溴马隆提供了一个简单、快速、灵敏的方波溶出伏安（OSWSV）检测方法，并成功的应用于模拟血清样及尿样中胺碘酮及苯溴马隆的检测，获得较为满意的结果。论文的第四章详细研究了芳胺类药物：盐酸普鲁卡因及盐酸普鲁卡因胺在Nafion修饰玻碳电极上的电化学行为，建立了一种选择性好，灵敏度高的Nafion修饰电极微分脉冲溶出伏安法测定盐酸普鲁卡因及普鲁卡因胺的分析方法，使得二者的检测限接近nM级，同时将该体系应用于模拟血清样中普鲁卡因及普鲁卡因胺的检测，结果良好。并结合环境扫描电镜（ESEM）法，计时库仑，库仑电解等方法对普鲁卡因及普鲁卡因胺在Nafion修饰电极上的电化学氧化反应机理进行了探讨，测定了两物质在Nafion膜上的扩散系数、选择性系数和分配系数。毛细管电泳-电化学检测技术对具有电化学活性的物质的分离检测具有极大的优越性。在第四章对结构极为相似的普鲁卡因及普鲁卡因胺进行详细的电化学研究后，论文的第五章采用高效毛细管电泳－安培检测法对普鲁卡因及普鲁卡因胺同时进行分离检测，13min内完全分离。该法重现性好，灵敏度高，线性范围宽，用于模拟血清样中普鲁卡因及普鲁卡因胺的分离检测，获得较为满意的结果。更多还原

【Abstract】 According to World Health Report published in 2000, there were seventeen millions of people died of cardiovascular diseases, in another word, there was one third people died of cardiovascular diseases. Cardiovascular diseases become the large burden of world health care. Cardiovascular medicine mainly includes cardiotonic, antiarrhythmic drugs, antihypertensive drugs and antianginal drugs etc. In order to achieve the optimum curative effect, assure patient to take medicine safely and reasonably, some simple detection methods with high sensitivity and specificity for the Cardiovascular medicines should be developed. In this thesis, some detection methods for four kinds of cardiovascular medicines such as amiodarone, benzbromarone, procainamide and procaine hydrochloride are developed.In section 2 and section 3, the electrochemical behavior of amiodarone and benzbromarone was investigated by cyclic voltammetry, linear sweep voltammetry chronocoulometry and bulk electrolysis with coulometry on a glassy carbon electrode. A simple, quick and sensitive method for detection of amiodarone by differential pulse voltammetry and a simple, quick and sensitive method for detection of benzbromarone by Osteryoung Square Wave Stripping Voltammetry were developed. The methods were applied to the assay of amiodarone and benzbromarone in rabbit serum and urine with good results.In section 4, the electrochemical behavior of some aromatic amine compounds such as procainamide and procaine on a Nafion modified electrode was investigated. A high selective and sensitive differential pulse voltammetry method for determination of procainamide and procaine with a low detection limit in the sub nM range was developed. This method was applied to the assay of procainamide and procaine in rabbit serum with good results. The ion-exchange selectivity coefficient, the apparent diffusion coefficient and the distribution coefficient were determined based on the data from experiments of chronocoulometry, bulk electrolysis with coulometry etc. Since capillary electrophoresis with electrochemistry detection has great<WP=4>superiority for separation and assay of electrochemistry active materials, and based on the discussion of the electrode reaction mechanism of procainamide and procaine in section 4, a capillary electrophoresis with amperometric detection was employed for the separation and determination of procainamide and procaine in section 5. Under the optimum conditions, the two analytes were separated completely within 13 min. The method has been employed satisfactorily for the determination of procainamide and procaine in rabbit blood by standard addition with good reproducibility and high sensitivity.更多还原