【作者】 陈海川；

【导师】 徐兰；

【作者基本信息】 四川大学 ， 无线电物理， 2003， 硕士

【摘要】 细胞膜是细胞外大分子进入细胞内的主要屏障，电穿孔(Electroporation)现象是指在电磁脉冲(Electromagnetic Pulses，EMP)作用下，细胞膜两边产生感应电位，破坏了细胞膜的绝缘性，从而使细胞膜的稳定性降低，瞬时增强细胞膜的通透性，在细胞膜的双脂层上形成瞬时可逆微孔的生物物理现象。电穿孔现象创造了一条通向细胞内的通道，可促进抗癌药物等大分子进入细胞内部，这种利用电穿孔现象将抗癌药物导入细胞内的治疗肿瘤的方法被称为肿瘤的电穿孔治疗。 在本论文中，我们叙述了细胞膜电穿孔的过程，通过实验的方法观察到了HeLa等细胞在电磁脉冲作用下的电穿孔现象，从实验总结获得了细胞膜电穿孔的最佳电磁脉冲参数；我们研究并报道了电磁脉冲对治疗肿瘤的作用，在实验装置、实验方法等方面都进行了有益的探索，并取得了良好的实验结果，研究结果得到同行专家关注与认可，实验结果处于国内研究的前列。为进一步的动物实验研究以及临床肿瘤治疗的研究提供了可供参考的方法和数据。我们发现：通过小动物实验，在电磁脉冲和抗癌药物的共同作用下，接种在小动物身上的肿瘤能够得到有效的抑制或治愈，肿瘤的完全响应率达到65％，部分响应率为10％，可见治疗效果是很明显的。本论文分为四个部分，第一部分为在电磁脉冲作用下细胞的电穿孔效应、原理和机制，第二部分叙述了电磁脉冲导致细胞膜电穿孔的实验研究，第三部分研究了电磁脉冲在治疗肿瘤中作用，包括实验过程和基本结果。第四部分总结了电穿孔治疗的现状及其展望。更多还原

【Abstract】 Cell membranes are the mainly barrier for the exchange of molecules between the ceil interior and exterior. When cells are subjected to the electromagnetic pulse (EMP), the membrane permeability barrier is temporarily suppressed and the membrane stability is decreased, this was termed electroporation. Electroporation is the physical process of inducing transient permeability of cell membranes by EMP, so Electroporation provide a physical method for entering the cell interior, and increase the anti-cancer drugs into the cell interior greatly.In this paper, we reported that the process of electroporation, and observed the electroporation of HeLa by experiment, and got the perfect parameters of EMP for the electroporation; on the other hand, we reported a new approach for the treatment of sarcoma-Electroporation therapy (EPT). Electroporation can accelerate molecules like anticancer drugs into cytoplasm by electromagnetic pulses (EMP). The EPT are used to treat the S180 sarcomas of the KunMing mice by intratumoral injection of low dosage of drugs. From the experiment, we discovered that this approach could obtain remarkable effect The paper is divided into four parts, in the first part, we described the principles of electroporation; in the second part, we described the electroporation of cells induced by the EMPs; in the third part, we studied the method of EPT, and got some results; the fourth part is the summary and outlook.更多还原