【作者】 曾云英；

【导师】 王永清；

【作者基本信息】 四川农业大学 ， 果树学， 2003， 硕士

【摘要】 本研究以金花梨（Pyrus pyrifolia Nak. cv. Jinhua）优系14#、18#、4#、3#的茎尖和试管苗叶片为试材进行营养器官培养研究，实验采用正交实验设计并结合SPSS统计分析软件包对实验结果进行分析，着重研究金花梨优系茎尖再生植株无菌培养物建立、初代培养、增殖培养和生根培养四个环节和诱导叶片产生愈伤组织，结果表明： 1)外植体最好的消毒方式是：剥去芽外层鳞片，只留有2—3个叶原基，用0.1％生汞+适量洗衣粉消毒5分钟，无菌水冲洗4—5次。 2)初代培养：芽萌发培养基为1/2MS+6-BA1.5mg/l+IBA0.1mg/l+GA₃l～3mg/l可获得良好的培养效果。 3)增殖培养：培养基为MS+6-BA1.5mg/l+IBA0.2mg/l，30～40天即增殖一代，平均每代增殖倍数大于3，年理论增殖系数可达5.3×10⁵。 4)增殖培养基中加入GA₃能明显促进外植体增殖和伸长。随着GA₃浓度的升高，外植体的增殖倍数和伸长长度增加，在GA₃3.0mg/l时，增殖倍数达到6.47，茎伸长到3.90cm，且苗生长正常。 5)无菌生根培养：培养基为1/2MS+IBA0.5mg/l+活性炭0.1％，生根后转入无激素的1/2MS培养基中培养，生根苗长势较好，生根率和根数增加。 6)叶片培养：培养基为NN69+细胞分裂素（6—BA：KT：ZT=2：1：1）5.0mg/l+IBA0.3mg/l，愈伤组织诱导率可达87.25％。 7)不同优系茎尖培养表现不同，3#表现最好，分化率和增殖倍数最高，18#次之，4#表现较差，14#最差。更多还原

【Abstract】 The terminal buds and axillary buds and leaves were used for explants in the study of in vitro culture to establish a rapid technique system of micropropagation . Orthogoal design and SPSS for Windows statistical analysis software were employed in the experimentation. The results were as follows:1) Higher livability was obtained by stripping out squama, remaining two or three leaf anlage, sterilizing for five minutes in 0.1% Hgcl2 and some laundry powder, washing four or five times with asepsis water.2) Iniation medium: MS/2 (macroelement), MS (microelement), iron salts and organic substances with agar 0. 55%, sucrose 3%, 6-BA1. 5mg/l, IBA0. 1mg/l, GA31 -3mg/l was suitable for shoot growth and differentiation3)Multiplication culture: MS with agar 0.55%, sucrose 3%, 6-BA1.5 mg/1, IBAO. 2 mg/1 was suitable for multiplying buds. Theoretically, the rate of propagation of a shoot plumule could reach about 3 in 30-40 days and 5. 3x105 per year.4) Multiplication and the length of the stems were induced by adding some dose GA3. Multiplication coefficient could reach 6.47 and the length of stems could reach 3. 9cm by adding GA3 3. 0mg/l.5)Rooting:Roots were induced in MS/2+ agar 0.50%+sucrose 3%+IBAO. 5mg/l +NAAO. 3-0. 5mg/l+0. 1%AC, and the rooted plantlets were transferred into 1 /2MS.6)Leaf culture medium: NN69+ agar 0. 55%+sucrose 2% + 6 ?BA3. 0mg/1+ IBA0. 3mg/l, the rate of callus induction being 87.25%.7)Different lines of mother plants reacted differently.更多还原