【作者】 陈立新；

【导师】 俞汝勤； 曾鸽鸣；

【作者基本信息】 湖南大学 ， 分析化学， 2003， 硕士

【摘要】 本论文合成了几种含末端双键、可共价固定的新型荧光化合物，并以此作为荧光指示剂，与膜基质单体在光引发剂作用下发生光聚反应共聚在经过硅烷化处理的石英或普通玻片上制成光极膜，制备了对一些物质有响应的荧光化学传感器。（1）合成了N-烯丙基咔唑，研制了一种强力霉素荧光传感器，线性范围为6.0×10^-7-2.0×10^-3mol·1^-1，一些常见的无机盐和有机物对测定无明显干扰，测定结果与分光光度法测定的结果一致，研制的强力霉素传感器可测定药片和尿样中强力霉素的含量。（2）用荧光基团2-氨基苯并噻唑与对烯丙氧基苯甲醛合成了一种新的Schiff碱类化合物，研制成了一种Schiff碱新颖光化学传感器，用于呋喃唑酮的测定。检测范围为1.0×10^-6-1.0×10^-3mol·1^-1。用传感器测定药片中呋喃唑酮含量的平均值和标准偏差与药典中的标准方法分光光度法测定的结果一致。（3）用强荧光基团2-氨基苯并蒽酮与对烯丙氧基苯甲醛合成了另一种新的Schiff碱类化合物，与甲基丙烯酸羟乙酯、丙烯酰胺、环乙二醇二丙烯酸酯共聚制得光极膜，制备了一种苯并蒽酮Schiff碱光化学传感器，可用于单质碘的测定。测定时的最佳pH为8.0。单质碘猝灭光极膜的线性范围在1.0×10^-5-1.0×10^-3mol·1^-1之间，常见的无机离子和一些可能共存的有机物不干扰测定，用该传感器测试了普通食盐中碘的回收率。（4）合成了一种具较强荧光的的有机光致变色化合物4-甲基-7-烯丙基萘并[1，2-b]吡喃-2-酮，并将其作为荧光指示剂应用到光化学传感器中，研制了一种测定呋喃西林的荧光传感器，线性范围为6.0×10^-6-8.0×10^-4mol·1^-1。研制的呋喃西林传感器可测定药片中呋喃西林的含量。更多还原

【Abstract】 In this thesis, several new fluorescence carriers have been synthesized bearing a carbon chain with a terminal double bond introduced into the molecule and copolymerized with a monomer under UV irradiation on the silanized glass or quartz disk. The sensors prepared were studied. Covalent immobilization effectively prevents the leakage of the carrier dye from the sensor membrane, a phenomenon that shortens the lifetime of ordinary optical sensors. These sensor prepared have been applied to analysis of different species.(1) Carbazole is a promising pharmaceutical species. A novel optical sensor for determining doxycycline based on the fluorescence quenching of N-allylcarbazole immobilized on an quartz glass plate surface by covalent bonding has been described. The sensor shows satisfactory virtues in reversibility, repeatability, selectivity and sufficient lifetime resulting from its excellent optode membrane. Its response time is less than 60 seconds. The determination range and detection limit of the sensor are 6.0+10-7 2.0+10-3 mol.1-1 and 2.0+10-7 mol.1-1, respectively. The lifetime of each sensor is at least three to four months. The sensor can be used for direct determination of doxycycline in pharmaceutical preparations and urine samples.(2) An aminobenthiazole schiff base has been synthesized as a new fluorescence carrier by reacting 2-aminobenthiazole with p-allyoxybenzoldohyde. The new kind of schiff base can be utilized for preparing an optical sensor, used for determining furazolidone based on the fluorescence quenching. Aminobenthiazole schiff base immobilized on a glass plate surface by covalent bonding can circumvent the leakage of the fluorephore from the sensor surface and guarantee a relatively long working lifetime. The sensor shows satisfactory virtues in reversibility, repeatability, selectivity and sufficient lifetime. Its response time is less than one minute. The determination range and detection limit of the sensor are 1.0+10-6-1.0+10-3mol.1-1 and 6.0+10-7 mol.1-1. respectively. The sensor can be used for direct determination offurazolidone in pharmaceutical preparations and urine samples.(3) Another schiff base was synthesized by reacting 2-aminobenzanthrone with p-allyoxybenzoldohyde and used for determination of iodine based on fluorescence quenching. As a fluorescence carrier, the aminobenzanthrone schiff base was immobilized on a glass plate surface by covalent bonding. The sensor possesses a short response time, nice reproducibility and reversibility. Iodine in solution can be determined in range between 1.0+10-5 and 1.0+10-3 mol.1-1. The determination limit of the sensor is 6.0+10-6 rnol.l-1’. The sensor has been applied to the dettermination in sodium chloride.(4) 4-methyl-7-allyloxynaphtho[l, 2-b]pyran-2-ketone has been synthesized as a fluorescent carrier for preparing optical chemical sensor and used for direct determination of nitrofurazone. The carrier is immobilized on a quartz glass plate surface treated with a silanizing agent to prevent the leakage of the dye. This sensor can be utilized for nitrofurazone assay based on fluorescence quenching. The sensor shows good repeatability, long lifetime and a fast response of less then 2.5 min. Nitrofurazone can be determined in the range between 6.0+10-6 to 8.0+10-4mol.1-1’ with a detection limit of 4.5+10-6 mol.1-1 at pH 6.0.更多还原