微波细胞吸收谱测量及生物效应研究

【作者】 罗鸣；

【导师】 刘盛纲； 余国芬；

【作者基本信息】 电子科技大学 ， 生物医学工程， 2002， 硕士

【摘要】 近年来，随着微波在工农业生产、医疗卫生、科学研究、通信广播及家用电器等各方面的广泛应用，电磁辐射与人类健康的关系得到了广泛的关注。人们对电磁场生物效应的研究也越来越广泛。本文介绍了目前国际国内该领域的研究现状；总结归纳了研究较多的电磁场生物效应，如增长率效应、电穿孔效应、对癌细胞的影响、大功率毫米波的生物效应等。对电磁场生物相互作用的热效应和非热效应的机理作了阐述，分析了国际上研究讨论较多的相干电振荡机理、场力效应机理，比较了热效应和非热效应之间的异同点。同时还讨论了微波辐射的防护及微波辐射标准的制定。围绕细胞微波吸收谱的测量，建立了扫频微波细胞吸收谱测量系统。该系统能对细胞微波吸收谱自动测量，并能对数据进行存储以便于分析比较。针对人鼻咽癌CNE₁细胞进行了吸收谱的测量。在同一功率密度下，进行了人鼻咽癌CNE₁细胞经不同频率微波辐照后生长及集落形成。就测量得到得最大和最小吸收功率进行了生物效应的初步研究。在此基础上分析了不同频率微波照射人鼻咽癌CNE₁细胞抗癌性的一些差异。针对实验过程、实验结果对提出了电磁场生物效应实验中要特别注意的问题，并对实验数据的准确性进行了分析，提出了改进意见。更多还原

【Abstract】 With microwave being extensively used in industryN agriculture> medical treatment x scientific research > communications and family electronic equipment, etc, the relation between electromagnetic radiation and human health is given more attention. This thesis describes researches in this field. Some commonly interesting bio-electromagnetic phenomenon are introduced, first, the growth rate effects, the phenomenon of "electroporation", the affect of microwave radio on cancer cell. The mechanism of electromagnetic thermal effect and non-thermal effect and them difference are given some theoretic models are summarized. It analysis some fashion mechanisms in the academe. A complete auto-test system for measuring microwave absorption of biological cells is built up. Absorption spectrum of nasopharyngeal cancer cell(CNEi) are measured. The results and discussions are given. According to the absorption measurement, some frequencies, i.e. the maximum and minimum absorption frequencies and a random frequency between them are selected to observe the biological effect, i.e. colony forming ability after exposing. The results are given and discussed. Finally, a summary and suggestions for similar measures are given.更多还原