【作者】 徐晔珊；

【导师】 肖国强； 邓树勋；

【作者基本信息】 华南师范大学 ， 运动人体科学， 2002， 硕士

【摘要】 本实验主要研究急性递增负荷运动条件下机体免疫的应答特征和恢复特征，并且，在恢复期以吸氧为辅助手段，观察吸氧对急性运动后免疫机能的影响。将实验对象分为吸氧组和对照组，测得两组安静时、运动即刻、恢复30分钟和恢复24小时的免疫指标的变化，包括红细胞免疫功能指标，即红细胞补体受体花环率(RBC-C_3bRR)和红细胞免疫复合物花环率（RBC-ICR）；免疫球蛋白IgG、IgA、IgM，并对上述指标的变化趋势作了分析。并且将急性递增负荷运动后血清MDA和血乳酸的变化与免疫指标的变化进行相关性分析，以探讨这种运动条件下免疫机能应答变化和恢复变化的可能机理。同时，将两组的各个指标比较分析，试图为吸氧在运动恢复中的应用提供实验依据。 20名体育学院健康男生随机分为两组（吸氧组和对照组），实验要求全体受试者完成一次性递增负荷运动，运动至力竭，观察运动即刻和恢复期各指标的变化情况。 结果表明： 1．急性递增负荷运动即刻，RBC-C_3bRR明显下降，RBC-ICR明显上升，红细胞免疫功能呈继发性下降。结果表明急性大强度运动即刻，红细胞免疫功能暂时抑制，与急性运动后白细胞免疫功能的变化趋势基本一致。提示，运动过程中，红细胞免疫与整体免疫机能的变化关系密切，是反映运动应激影响免疫功能的重要组成部分。 2．急性递增负荷运动恢复期机体免疫机能的变化规律如下：运动后恢复30分钟时，红细胞免疫功能增强，免疫抑制解除，红细胞免疫粘附活性高于安静值，24小时后恢复正常。表明此种运动条件下，红细胞免疫功能经历激活阶段、补偿阶段、复归阶段的变化规律。 3．比较吸氧组和对照组在运动后恢复期各免疫指标的变化，发现吸氧能在一定程度上减轻急性运动后的免疫抑制，促进运动后免疫机能的恢复。 — —df—— 主要反映在红细胞免疫功能的变化，吸氧组RBC－C3bRR在恢复30分钟 时上升更明显，同时＊＊＊－IC R下降也更明显(吸氧组P＜队05，对照组 P＞O0引，且恢复N小时下降至安静值以下。提示，运动后吸氧可能加快 消除运动疲劳，于运动恢复有一定的应用价值。 4．实验观察了运动过程中吸氧对红细胞免疫功能的影响，发现运动恢复 期，吸高浓度氧能增强红细胞免疫粘附能力，加快红细胞对循环免疫复 大物的消除作用。 5．吸氧组和对照组血乳酸的变化并没有显著性差异，提示，吸氧的恢复 作用与吸氧浓度和吸氧时间密切相关，积极发挥吸氧在运动后的恢复效 用可能存在某种有效方案，有待于进一步研究。 6．急性递增负荷运动后免疫球蛋白的总体变化趋势趋于稳定，与多数研 究的结果相符。吸氧对兔疫球蛋白的恢复产生一定的影响，但运动后吸 氧与体液免疫的具体情况有待于进一步实验证实。更多还原

【Abstract】 This study tried to observe responsive and recovery characteristics of immune function induced by an acute incremental ergometer exercise, and to observe the effect of hyperoxia intake on immune recovery rate.20 students from SCNU served as the subjects, and they were randomly divided into the hyperoxia group and the control group. The venous samples were taken before exercise, just after exercise, post-exercise SOmin and 24h, respectively. The immune parameters included RBC-CsbRR, RBC-ICR, immunoglobulins ( IgG, IgA and IgM). The correlation of serum MDA and LA with these parameters were analyzed so as to observe the tendency of immune alteration after acute incremental exercise and to understand if hyperoxia intake is helpful to accelerate recovery rate of exercise-induced fatigue.Subjects of two groups executed an same incremental ergometer exercise up to exhaustion. After exercise, compared with the natural recovery in the control group, SOmin hyperoxia (70% oxygen) was given to the experiment group.The results as follows:1. RBC-CabRR of two groups decreased significantly just after exercise and RBC-ICR showed reverse change. This indicated that the erythrocyte immunity was suppressed just after acute incremental exercise.2. Compared to just after exercise, RBC-CsbRR increased significantly SOmin after exercise, and returned basically to pre-exercise level 24 hours later. Otherwise, RBC-ICR showed a continuous fall SOmin and 24 hours after exercise, and returned to pre-exercise level 24 hours later. It indicated that the erythrocyte immune function showed an enhanced state SOmin after exercise and had basically restored 24 hours after exercise. As a thumb rule, responsive course of the erythrocyte immunity to acute incremental exercise could be divided into three phases: activation, compensation and restoration.3. The difference of immune parameters between hyperoxia group and control group was observed so as to determine if hyperoxia intake could facilitate eliminating rate of immunosuppression. Compared with control group, RBC-CabRR in hyperoxia group showed more significant increase and RBC-ICR more significant decrease., up to 24 hours later, even lower than pre-exercise level. It suggested that hyperoxia intake, to some extent, may accelerate the recovery rate of immunosuppression after acute exercise, and that hyperoxia intake can serve as a method to eliminate exercise-induced fatigue.4. Effects of hyperoxia intake on the erythrocyte immunity was observed especially after exercise. The results indicated that the ability to immune adhesion of erythrocyte showed increase after hyperoxia intake recovery, and that eliminating rate to immune complex raised more significantly,.5..Compred with control group, the serum MDA, LA has similar alterative tend in experiment group after hyperoxia intake. Such results indicated that how to apply this method effectively after exercise maybe determined by intake time, which should be studied further.6. Consistent with most research, immunoglobulin level changed insignificantly after acute exercise. The results showed the recovery rate of immunoglobulin changed after hyperoxia intake, however, whether should be study further.更多还原