【作者】 蒋慧萍；

【导师】 李杨瑞；

【作者基本信息】 广西大学 ， 作物栽培学与耕作学， 2002， 硕士

【摘要】 本实验选用五个不同地理来源(广西品种、海南品种、非洲品种、缅甸品种和印度本地品种)木豆品种和三个ICRISAT杂交木豆品种(ICPH8、ICPL87091、ICPL87119)作为供试材料。一方面，测定了不同品种的光合特性、叶绿素含量、束缚水／自由水的比值和丙二醛含量，同时还进行了一年的田间指标的调查；另一方面，本实验分别采用了形态标记、过氧化物同工酶标记和RAPD标记对八个品种的遗传多样性进行了研究，并对形态标记及RAPD实验结果进行了聚类分析，结果表明： 1．ICPL87091的光合速率(Pn)和叶绿素含量均显著高于其它品种，说明该品种具有良好的光合生理功能，其次是广西品种。 2．缅甸品种、广西品种和印度本地品种的束缚水／自由水比值均比其它试验品种高，而丙二醛含量均低于其它品种，表明它们具有相对较强的抗旱性能，但海南品种则相反。 3．通过对过氧化物酶同工酶酶谱分析，发现8个木豆品种的电泳图谱谱带条数和迁移距离相同，只是部分谱带存在颜色深浅的差别。 4．通过对八个品种一年的表型性状观察，发现各品种在表型上存在一定差异，如生育期、花色和粒色等。将表型的18个数量性状进行聚类分析，发现各品种间的遗传相似系数高；同时对八个品种进行RAPD标记，经聚类分析，同样也得到各品种间的遗传距离很近，平均为0.25。从而证实了八个木豆品种遗传基础接近，具有较近的亲缘关系。更多还原

【Abstract】 Five different pigeonpea varieties from different areas (i.e. Guangxi, Hainan, Africa, Burma and India ) and three hybrids bred by ICRISAT were investigated. Pn, Chi and MDA contents, and the ratio of bound-water/free-water were determined. The biological characters were investigated in the field. And the biological morphological markers, peroxidase isozymes and RAPD techniques were used for clustering the varieties.The results showed as follows:1. Pn and Chi contents in ICPL87091 were higher than any other varieties remarkably. It indicated a considerable photosynthetic capacity in ICPL87091. The following was the Guangxi variety.2. The ratio of bound-water/free-water in Burma, Guangxi and Indian varieties were higher than any others, but the content of MDA in these varieties was lower than 5 other varieties. It showed that the drought resistance in these varieties was stronger than any other varieties. However, the Hainan variety showed opposite.3. The result of peroxidase isozyme showed that the numbers of electrophoresis profile and the moving distance were the same in 8 different varieties, except for the color deepness in the profiles.4. Base on the observation on the 8 pigeonpea varieties for one year, some differences were detected among these varieties, such as growth duration, flower color and seed color etc. The results of cluster analysis in 18 quantity characters demonstrated that there was a high proximity between varieties. The cluster analysis of RAPD data showed the similar results, and the average genetic distance is 0.25. It further proved that they had a narrow genetic basis, and showed a close genetic relationship.更多还原