刺果甘草化学成分及抗肿瘤活性研究

【作者】 石荣火；

【导师】 阚毓铭；

【作者基本信息】 南京中医药大学 ， 中药学， 2001， 硕士

【摘要】 从豆科甘草属植物刺果甘草（Glycyrrhiza pallidiflora Maxim）根及根茎中分出17个化合物（Ⅰ～ⅩⅦ），根据理化性质和UV、IR、EIMS、¹HNMR、¹³CNMR及DEPT等波谱数据确定了其中13个化合物的结构，分别为：后莫紫檀素（Homopterocarpin，Ⅲ）、5α-豆甾-3，6-二酮（5.α-Stigmastane-3，6-dione，Ⅴ）、β-谷甾醇（β-Sitosterol，Ⅵ）、4’，7-二甲氧基异黄酮（4’，7-Dimethoxyisoflavone，Ⅶ）、白桦脂酸（Betulinic acid，Ⅷ）、美迪紫檀素（Medicarpin，Ⅸ）、异光甘草酚（Isoglabrol，Ⅺ）、10-甲氧基美迪紫檀素（10-Methoxy-medicarpin Ⅻ）、9-甲氧基香豆雌酚（9-Methoxycoumestrol，ⅩⅢ），21α-hydroxy-3-oxo-oleana-11，13（18）-diene-29-oic acid（ⅩⅣ），芒丙花素（Formononetin，ⅩⅤ）、马其顿酸（macedonic acid ⅩⅥ）、胡萝卜甙（Daucosterol，ⅩⅦ）。其中ⅩⅣ为一新化合物，Ⅴ和Ⅻ为甘草属中首次分得，Ⅷ，ⅩⅦ为首次自该植物中分得。 采用小鼠体内接种实体HepA瘤株，观察了后莫紫檀素、美迪紫檀素等7个单体对HepA实体瘤的抑制作用，结果发现，其中5个单体有较强的活性（抑瘤＞40％），对其深入评价工作正在开展。更多还原

【Abstract】 Seventeen compounds were isolated from roots of Glycyrrhiza pallidiflora Maxim, a Chinese folk medicine .Among them, thirteen compounds’ structures were established as (-) -6aR, 11 aR-dihydro-3,9-dimethoxy-6H-benzofuro [3,2c] [l]-benzopyran (homopterocarpin III) ,5.alpha.-stigmastane-3,6-dione (V),stigmast-5-en-3 P -ol O-Si tosterol VI), 7-methoxy-3-(4methoxypheny1)-4H-benzopyran 4-one (4’, 7-dimethoxyisoflavone W) , 3 ?-hydroxy-lup-20(29)-en-28-oic acid (betulinic acid VI) , (-)-6aR,llaR-dihydro-3-hydroxy-9-methoxy-6H-benzofuro[3,2c] [1] -benzopyran(medicarpin IX), (s) 2,3- dihydro-7- hydroxy-2-(4-hydroxyphenyl)-6, 8-bis-(3-methyl-2-butenyl)- 4H-benzopyran-4-one (isoglabrol XI) , (-)-6aR,naRKiihydro-3-hydroxy-9,l(klimethoxy-6H-tenzofuro[3,2c] [1]-benziopyran (10-methoxy-medicarpin XH) ,3-hydroxy-9-methoxy-6H-benzofuro[3,2c][l]benzopyran-6-one (9-methoxycoumestrol XIII), 21 a -hydroxy-3-oxo-oleana-ll, 13(18)-diene-29-oic acid (XIV), 7-hydroxy-3~ (4-methoxyphenyl)- 4H-benzopyran-4-one (formononetin XV), 3 ^ , 21 a-dihydroxy-oleana-11, 13(18)-diene-29-oic acid (macedonic acid XVI) and stigmast-5-en-3 3-D-glucopyranoside (daucosterol XVII) respectively,byspectroscopic methods. Of them, XIV was a new oleanane triterpenoid, V and Xfl were firstly reported in Glycyrrhiza genus, and other tow compounds , Vffl and XVfl were firstly founded in this plant.The antiHepA tumour effects were observed in mice administrated seven compounds of above mentioned, five of them showed significant antiHepA activity . Further investigations are now in progress in our laboratory.更多还原