上颌窦鳞状细胞癌组织中p27蛋白和增殖细胞核抗原表达及其临床意义

【作者】 韩敏；

【导师】 张秋贵；

【作者基本信息】 青岛大学 ， 耳鼻咽侯科学， 2001， 硕士

【摘要】 为探讨上颌窦鳞状细胞癌与抑癌基因p27和增殖细胞核抗原（PCNA）表达及其临床意义，对上颌窦鳞癌35例，其中高分化鳞癌12例、中分化鳞癌10例、低分化鳞癌13例，鼻腔鼻窦内翻性乳头状瘤15例进行了研究。本文采用免疫组化方法检测了上颌窦鳞癌和内翻性乳头状瘤组织中p27蛋白和PCNA的表达，并与HE染色方法相比较。结果显示，p27蛋白在上颌窦鳞癌组病人中阳性表达率为25.71%（9/35），而在对照组鼻腔鼻窦内翻性乳头状瘤组中的阳性率为73.33%（11/15），两组之间差异有极显著意义（P＜0.01）。但在上颌窦鳞癌高、中、低分化三组之间差异无显著意义（P＞0.05）。PCNA指数在两组病变间存在极显著性差异（P＜0.01），而且不同分化程度上颌窦鳞癌的三组间PCNA指数亦有显著性差异（P均＜0.05）。p27蛋白阳性与p27蛋白阴性病人的PCNA指数差异有显著性（P＜0.01）。结果表明，p27和PCNA检测有助于更准确地估计上颌窦鳞癌及内翻性乳头状瘤的生物学特性，更有效的反映了上颌窦鳞癌的增殖活性。两个指标综合检测有助于上颌窦鳞癌的诊断，有助于上颌窦鳞癌恶性程度的估计，为上颌窦鳞癌的预后判断和临床治疗提供参考。更多还原

【Abstract】 In this study, p27 protein and PCNA in maxillary sinus squamous cell carcinoma (MSCC) were examined with immunohistochemical method, and compared with HE staining. We tried to found out the relationship between MSCC and the expression of p27 protein and PCNA, so as to find a way to help diagnose early the malignant tumour and make clear its degree of differentiation. Fifty paraffin sections of 50 patients were studied, among which were 35 maxillary sinus squamous cell carcinomas and 15 inverted papillomas of nasal sinus (NIP) as the control group. Among MSCC, there were 13 well differentiation, 10 moderate differentiation and 12 poor differentiation. All the samples were provided by the Department of Pathology of Affiliated Hospital of Qingdao University Medical College, and were diagnosed by at least two skilled pathologists.The rate of p27 protein positive staining in MSCC was 25.71% (well differentiated MSCC, 25.0%; moderately differentiated MSCC, 30.0%; poorly differentiated MSCC, 23.1%). Furthermore ，11 of 15 NIP showed p27 protein positive staining. Significant difference was found between MSCC and NIP (P＜0.01)，but there was no significance among well, moderately and poorly differentiated MSCC（P＞<WP=7>0.05）.MSCC had more PCNA positive cells than NIP. There was significant difference between random differentiated groups of MSCC（P＜0.05）.The results indicated the proliferating activities of benign or malignant tumours of maxillary sinus and various differentiated MSCC. The expression of p27 protein and the reduction of PCNA labeled index(PCNA LI) had significant difference（P＜0.01）.The study proved that p27 and PCNA could help evaluate the clinicopathological characteristics of MSCC and NIP, and they can be important factors to reflect the proliferating activity of MSCC. The two factors confined the evidences to predict the prognosis and treat the disease.更多还原