库拉索芦荟组织培养技术体系研究

【作者】 杜文平；

【导师】 石大兴；

【作者基本信息】 四川农业大学 ， 森林培育学， 2001， 硕士

【摘要】 本研究以库拉索芦荟（Aloe Vera Linme）的茎段进行组织培养技术体系的建立，通过正交设计结合方差分析、极差分析，着重研究适合库接索芦荟组织培养的最佳培养基配方及培养程序，在培养过程中的培养反应以及炼苗这一重要环节，结果表明： ① 将茎段用洗衣粉漂洗3分钟，流水冲洗30分钟，再用75％酒精和0.1％ 升汞分别消毒10秒钟和5分钟，可有效地扼制污染的发生； ② 初代培养：中等无机盐浓度的改良H培养基为最适基本培养基，用改 良H培养基+ZT2.0mg/L+BA0.5mg/L+NAA0.05mg/L+糖20g/L可达到初 代培养良好的启动效果； ③ 继代培养：培养基为MS+BA2.0mg/L+NAA0.01mg/L，30天时增殖系数 为4.17，而35天为芦荟最适继代周期，年理论增殖系数可达1.59× 10~6； ④ 生根培养：KC培养基+IBA0.3mg/L+活性炭0.2％+糖20g/L对芦荟生根 良好，生根率为95.83％，生根数为4.3条，平均根长为4.67cm； ⑤ 炼苗：有根芦荟炼苗成活率高于无根苗，用活性炭在瓶内生根的芦荟 比不用活性炭生根的芦荟炼苗易成活，炼苗适合的基质是珍珠岩：蛭 石=1：1。更多还原

【Abstract】 Study on technique system of tissue culture in Aloe Vera Du Wenping (College of Forestry and Horticulture, Sichuan Agricultural University, Ya抋n, Sichuan, China, 625014) Abstract The stem of Aloe Vera Linme was used for explant to establish the technique system of tissue culture. With Orthogonal design and analysis of variance, to find out the best medium and proceeding of culturing Aloe Vera. The results were as follows: ~Dstems ware sterilized in 75% alcohol and 0.1% mercuric chloride solution for 10 Seconds and 5 minutes well; ~ Iniation medium: H medium, exchanged well, with ZT2.Omg/L NAAO.O5mgIL and sugar 20g/L, is the best iniation medium; ?Subculture medium: The MS medium with the 2.Omg/L BA and 0.Olmg/L NAA was applied to the subculture medium, and the increasing coefficient in one month is 4.17, Using 35 days as subculture time, theoretically, the times of propagation is 1.59X 106 for one year; ~ Rooting culture: The rooting rate in the KC medium with 0.3mg/L IBM ACO.2% and sugar 2OgIL is 95.83%, the number of roots are 4.30, the length of root is 4.67cm; ~ Transplant: rooted Aloe or rooted Aloe in the rooting medium with AC or the transplant medium in perlite and vermiculite by 1 to 1, are all useful to root.更多还原