【作者】 王桂华；

【导师】 孙汉文； 鲍所言；

【作者基本信息】 河北大学 ， 分析化学， 2001， 硕士

【摘要】 本文共分五章。第一章为文献综述，对荧光共振能量转移技术的原理、分析技术及其应用进行了较全面的综述。引用文献56篇。 第二章对碱性荧光染料吖啶橙（AO）、吖啶黄（AY）、罗丹明6G（R6G）、罗丹明B（RB）、乙基罗丹明B（ERB）、丁基罗丹明B（BRB）、碱性藏花红（ST）组成的各种染料混合体系的能量转移进行了研究。研究了各种表面活性剂对碱性阳离子染料间能量转移的影响，以及转移效率的影响因素。 第三章介绍了荧光能量转移在测定无机离子中的具体应用。第一节吖啶橙—罗丹明6G能量转移荧光猝灭法测定痕量磷；第二节PAR—吖啶黄能量转移荧光猝灭法测定痕量铁（Ⅱ）的研究。 第四章罗丹明B-7-碘-8-羟基喹啉-5-磺胶束体系荧光熄灭法测定钒。 第五章苯基荧光酮荧光分光光度法测定粉煤灰中的痕量锗。更多还原

【Abstract】 This paper consists of five chapters. In the first paper, Application of fluorescence analysis based on resonance energy transfer (FRET)is reviewed with 56 references. The review includes Fster theory of fluorescence resonance energy transfer, analysis techniques and application. In the second chapter the fluorescence energy transfer between some basic dyes, such as acridine orange (AO), acridine yellow (AY), rhodamine 6G(Rh6G), rhodamine B (RhB), ethyl ester of rhodamine B (ERhB), buthyrhodamine B (BRhB) and safranine T (ST), was investigated. The effect of various surfactants on fluorescence energy transfer of basic dyes was studied, too. The conditions effecting energy transfer efficiency were discussed. Practical application of fluorescence energy transfer to the determination of inorganic ions was introduced in the third chapter. In the first section, the determination of trace phosphorus by using energy transfer fluorescence quenching from acridine orange to rhodamine 60 was studied. In the second section, study on the energy transfer florescence qtienching among combination of acridine yellow-PAR determination of trace iron. In the fourth chapter, determination of trace Vanadium(v) by a fluorescence quenching method with rhodamine B-7-iodo-8-hydroxyquinoline-5-sulfonic acid surfactant system was studied. In the fifth chapter, spectrofluorometric determination of trace germanium with phenylfluorone in flyash was investigated.更多还原