【作者】 杨修仕；

【导师】 董川； 任贵兴；

【作者基本信息】 山西大学 ， 环境科学， 2011， 硕士

【摘要】 第一章：介绍了大豆皂苷的结构、分类、常见生物活性以及大豆皂苷的提取、分离、纯化、检测方法,并对大豆皂苷的降解动力学进行了简单概述。同时对酒精性肝病、相关致病机理及其天然植物化学物治疗进行了综述。阐述了论文的立题背景、主要研究内容和创新点。第二章：利用高效液相色谱-质谱联用(HPLC-MS)对单体皂苷进行鉴定,通过比较质谱参数,鉴定出原料大豆皂苷中的十种单体皂苷分别为大豆皂苷Aa,大豆皂苷Ab,大豆皂苷Ae,大豆皂苷Bb,大豆皂苷Bb’,大豆皂苷Bd,大豆皂苷Be,大豆皂苷αg,大豆皂苷βg,大豆皂苷βa；利用高效液相色谱检测原料大豆皂苷中含有的少量大豆异黄酮,5种检测到的大豆异黄酮及其含量分别为大豆苷(1.15 mg/g)、黄豆黄苷(0.09 mg/g)、染料木苷(2.68 mg/g)、丙二酰大豆苷(1.24 mg/g)、丙二酰染料木苷(4.57 mg/g)。第三章：通过建立急性酒精性肝损伤动物模型,系统探讨了大豆皂苷对小鼠急性酒精性肝损伤的影响。结果表明：雄性ICR小鼠在大豆皂苷的干预下可阻止因酒精摄入而导致的血清谷草酰胺转氨酶(AST)、丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)和乳酸脱氢酶(LDH)的活力增强以及肝脏总胆固醇(TC)、甘油三酯(TG)和丙二醛(MDA)的含量增加。与酒精对照组相比,大豆皂苷干预组表现出正常的抗氧化体系,组内小鼠肝脏超氧化物歧化酶(SOD)、谷胱甘肽-s-转移酶(GST)和谷胱甘肽过氧化物酶(GPx)活力均在正常范围,且肝脏谷胱甘肽(GSH)含量有所增加。组织病理学观察结果表明,大豆皂苷干预可阻止酒精导致的肝脏脂肪变性坏死、炎症和肿大。综上所述,大豆皂苷对急性酒精性肝损伤具有保护作用。第四章：采用经典恒温法对大豆皂苷Bb进行降解动力学研究。将大豆皂苷单体Bb分别置于水浴锅(80℃、90℃、100℃)和反应釜(110℃、120℃、130℃)中加热,每隔10min取样考察大豆皂苷Bb的含量变化,利用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)进行含量测定；通过数学拟合,确定大豆皂苷Bb的降解符合一级反应动力学,运用Arrhenius方程,可求得大豆皂苷Bb的降解活化能Ea为31.71KJ·mol-1。更多还原

【Abstract】 Chapter 1:The structure, classification and common bioactivity of soyasaponins are reviewed in details. The extraction, separation, purification and detection for soyasaponins are summarized. Then the alcoholic liver disease (ALD) and its pathogenesis are depicted in brief, as well as the treatment of ALD by natural plant chemicals. At the end, the backgrounds, significance, main content, and the innovative points of this assay are stated.Chapter 2:The high performance liquid chromatograph-mass spectrometry (HPLC-MS) is used for the identification of soyasaponins. Ten soyasaponins in the soy saponins are identified as follows:soyasaponins Aa, soyasaponins Ab, soyasaponins Ae, soyasaponins Bb, soyasaponins Bb’, soyasaponins Bd, soyasaponins Be, soyasaponinsag, soyasaponinsβg, and soyasaponins (3a. In addition, the isoflavones in the soy saponins are determined by high performance liquid chromatograph (HPLC) and the result is as follows: daidzin (1.15 mg/g), glycitin (0.09 mg/g), genistin (2.68 mg/g), malonyldaidzin (1.24 mg/g), malonylgenistin (4.57 mg/g).Chapter 3:The protective effects of soy saponins (SS) against acute alcohol-induced hepatotoxicity were investigated in Institute of Cancer Research (ICR) mice. Administration of SS prior to alcohol significantly prevented the increases in mice serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) caused by alcohol, as well as hepatic triglyceride (TG), total cholesterol (TC), and malondialdehyde (MDA) levels. Mice treated with SS showed a better profile of the antioxidant system with normal superoxide dismutase (SOD), glutathione-S-transferase (GST), and glutathione peroxidase (GPx) activities, which were associated with the increase of hepatic glutathione (GSH) levels relative to the acute alcohol treated group. Supplement of SRE prevented alcohol-induced hepatic steatosis necrosis, inflammation and swelling, as indicated by liver histopathological studies. All these findings demonstrate that SS has protective effects on acute alcohol-induced liver damage.Chapter 4:The degradation kinetics of soyasaponin Bb is studied by classical isothermal kinetic method. Soyasaponin Bb is put into water bath (for 80℃,90℃,100℃treatment) and agitated reactor (for 110℃,120℃130℃treatment). The sampling process is carried out every 10 min and the content of soyasaponin Bb is determined by high performance liquid chromatograph-evaporative light-scattering detector (HPLC-ELSD). The degradation kinetics of soyasaponin Bb is in accord with the first order reaction by mathematical fitting. On this basis, the degradation activation energy (Ea) is calculated to be 31.71 KJ·mol-1 by the Arrhenius Equation.更多还原