【作者】 程浩；

【导师】 胡秋辉；

【作者基本信息】 南京农业大学 ， 食品工程， 2010， 硕士

【摘要】 蜂胶是蜜蜂从外界的胶源植物的嫩芽或树皮伤口上采集的树脂,并混入蜜蜂上颚腺物和蜂蜡而成的一种棕红色、棕黄色、棕褐色或带有青绿色的粘性固体。蜂胶有较高的营养价值和保健作用,很受消费者青睐。近年来,对蜂胶的有效成分和生物学功能的研究取得了很大的进展,人们已从蜂胶中分离鉴定出数百种活性成分。蜂胶作为一种多功能物质,已被开发成多种保健食品。蜂胶软胶囊的开发为蜂胶在功能性食品中应用奠定了一定的基础。因此,本研究对蜂胶软胶囊的增强免疫功能和毒理学性质进行了研究。在用不同剂量的蜂胶软胶囊灌胃小鼠30d发现,蜂胶软胶囊对试验前后小鼠体重和脏器/体重比值均无显著影响。在二硝基氟苯(DNFB)诱导的小鼠迟发性变态反应(DTH)和ConA诱导的小鼠脾淋巴细胞转化试验(MTT法)的细胞免疫功能试验中发现,中剂量的蜂胶软胶囊能够增强DNFB引起的小鼠迟发性变态反应作用,而高剂量的蜂胶软胶囊能够增强淋巴细胞增殖能力,说明蜂胶软胶囊可增强小鼠细胞免疫功能。在体液免疫功能试验中,三个剂量的蜂胶软胶囊对小鼠产生血清溶血素的能力均无显著性影响,但中、高剂量的蜂胶软胶囊能够显著增强小鼠产生抗体生成细胞的能力。单核-巨噬细胞功能试验中,三个剂量的蜂胶软胶囊对小鼠碳廓清能力无显著影响,但高剂量的蜂胶软胶囊能够显著增强小鼠腹腔巨噬细胞的吞噬能力(P<0.05)。中、高剂量的蜂胶软胶囊能显著增强小鼠NK细胞活性的作用。上述试验结果表明,蜂胶软胶囊具有增强免疫力的功能。采用急性毒性试验、遗传毒性试验(Ames试验、小鼠骨髓微核试验、小鼠精子畸形试验)和30d喂养试验等对蜂胶软胶囊的食用安全性进行毒理学试验评价,结果发现蜂胶软胶囊对雌雄小鼠体重并无显著影响；而且Ames试验、小鼠骨髓微核试验、小鼠精子畸形试验三项遗传毒性试验结果均为阴性；30d喂养试验未见试验大鼠的生长发育、血液学、生化、脏体比及组织病理学有异常变化。因此,蜂胶软胶囊急性毒性分级属无毒级、无遗传毒性。更多还原

【Abstract】 Propolis, as a brownish red, brownish yellow chocolate brown or green viscous solid, is a mixture of resins collected by honeybee from young bud or bark wound of propolis plants, beeswax and secretion from mandible. It is welcomed for its higher nutrition values and health function. In recent years, with a greate advance of studies on active compositions and biological functions of propolis, hundreds of components were isolated and identified from propolis. Propolis, as a multi-functions material, has been developed to many functional foods. The development of propolis soft capsule can lay a foundation for the utilization of propolis in functional foods. Therefore, Enhancing immune and toxicology of propolis soft capsule were researched in this study.Results of this study showed that propolis soft capsule had insignificant effect on the body weight and organ/body weight of mice after supplemented with propolis soft capsule for 30days. In cellular immunity assay, middle dose of propolis soft capsule could accelerate ConA-induced mice spleen lymphocytes trancformation and high dose of propolis soft capsule had a positive effect on DNFB-induced delayed type hypersensitivity (DTH) reaction, which suggests that propolis soft capsule can enhance cell immune function of mice. In humoral immunity assays, middle and high doses of propolis soft capsule significantly enhanced the ability of mice to generate antibody producing cells, but no effect on serum hemolysin content for all three doses of propolis soft capsule. In monocyte-macrophages assays, all three doses of propolis soft capsule had insignificant effects on carbon clearance rate (P>0.05), while high dose of propolis soft capsule significantly enhanced phagocytic function of abdominal macrophage. Middle and high doses of propolis soft capsule also significantly enhanced NK cell activity. All results above imply that propolis soft capsule can enhance immunity.Acute toxicity test, genetic toxicity test (Ames test, mouse bone marrow micronucleus test, and sperm shape abnormality test in mice), and 30 days feeding test were employed for the toxicological evaluation on edible safety of propolis soft capsule. Results showed that propolis soft capsule had no effect on body weight of mice, and negative results were found in Ames test, mouse bone marrow micronucleus test, and sperm shape abnormality test in mice. Meanwhile, No unwonted change in growth and development, biochemical index, and pathology in organ, body and tissue was found. Therefore, propolis soft capsule was considered to be non-toxic and non-genetic toxicity class.更多还原