【作者】 涂念；

【导师】 张勇慧；

【作者基本信息】 华中科技大学 ， 药物化学， 2009， 硕士

【摘要】 湖北民间草药大接骨丹为山茱萸科鞘柄木属植物有齿鞘柄木(Toricellia an-gulata Oliv. var. intermedia (Harms) Hu)的根、根皮、茎皮、叶及花,可治疗外伤骨折、跌打损伤、风湿关节痛、干血痨伤、产后腰痛、慢性肠炎、腹泻、扁桃腺炎等。该药在湖北分布较广,恩施民间多用其治疗风湿关节痛,疗效确切,具有广阔的开发前景。本实验对大接骨丹95%乙醇提取物的石油醚部位和正丁醇部位进行色谱分离,得到13个化合物。通过理化常数、各种波谱分析技术或直接与标准品对照,鉴定其中的12个化合物分别为:二(4,7,8-三羟基-3,5-二甲氧基苯丙基)-9-醚(1,新化合物)、豆甾醇(2)、a-3,4,5-四羟基-苯乙酸(3)、豆甾-5,11-二烯-3β-醇(4)、22,23-二氢豆甾醇(5)、β-胡萝卜苷(6)、26,27-dinorcholesta-8,14-dien-3β-ol(7)、十六烷酸(8)、十八烷酸(9)、α-D-吡喃葡萄糖(10)、β-D-吡喃葡萄糖(11)、4-羟基-3,5-二甲氧基苯甲醛(12)。其中化合物1为新化合物;化合物3为首次从天然植物中提取分离得到;化合物1、2、3、7、12均为首次从该植物中分离得到。对大接骨丹中分离得到的六个单体化合物1、2、5、6、7、13进行了与RA发生相关的免疫活性实验—Con A诱导大鼠脾细胞增殖反应实验。结果表明,与对照组比较由大接骨丹分离所得这六个单体化合物对Con A诱导大鼠脾细胞增殖均有不同程度的抑制作用,差异具有统计学意义(P < 0.05)。更多还原

【Abstract】 Toricellia angulata Oliv. var. intermedia (Harms) Hu is the root or flower of Toothedleaf Torricellia of Cornaceae, used as a folk medicine in Hubei province for surgical trauma,fracture,rheumatic arthritis,blood stasis and tabes, puerperal lumbago, chronic enteritis, diarrhoea and tonsillitis. Toricellia angulata Oliv. with its excellent effect is widely used to treat rheumatic arthritis in Enshi of Hubei province, and it have an extensive perspective of development.During our investigation of the chemical constituents of this plant, thirteencompounds have been isolated from the petroleum ether, n-BuOH-soluble porti-ons in the 95% ethanol extract. On the basis of spectral analysis or direct co- chromatographies with authentic samples, twleve of them were identified as Di-(4,7,8-trihydroxy-3,5-dimethoxyl phenylpropyl )-9-ether (1, new compound), Stigmasterol (2), a-3,4,5-tetrahydroxy benzeneacetic acid (3), Stigmasta-5,11- dien-3β-ol (4), 22,23-dihydrostigmasterol (5),β-daucosterol (6),26,27-dinorchol-esta-8,14-dien-3β-ol (7), Palmitic acid (8), Octadecanoic acid(9),α-D-glucopy-ranose (10),β-D-glucopyranose (11), 4-hydroxy-3,5-dimethoxyl benzaldehyde (12). The compound 1 was a new compound, compound 3 was isolated from nature plant for the first time and compounds 1, 2, 3, 7, 12 were isolated from thisplant for the first time.Compounds 1, 2, 5, 6, 7, 13 were studied var experiments of immunological activity related to the creation of rheumatic arthritis—Con A-induced cell proliferation of rats splenocytes. Compared with the control group, those compounds have immunological inhibiting effect obviously on Con A-induced cell proliferation of rats splenocytes(P < 0.05).更多还原