【作者】 肖望鹏；

【导师】 顾晓英；

【作者基本信息】 宁波大学 ， 渔业资源， 2010， 硕士

【摘要】 本项研究旨在通过现场观测与实验室模拟实验了解三疣梭子蟹土池育苗对沉积环境产生的影响。测定了1期蚤状幼体（Z1期）阶段以及大眼幼体（M期）末期三疣梭子蟹育苗土池沉积物-水界面无机氮、活性磷的交换通量;通过实验室模拟实验,分析了pH、温度、氧化还原环境以及生物扰动作用对育苗池沉积物-水界面营养盐交换通量的影响;利用本实验室自行设计的无扰动底泥采集器采集沉积物样本,检测了各类无机氮、活性磷在底泥间隙水中的垂直分布;通过对不同育苗年限的土池沉积物进行总有机碳（TOC）、总氮（TN）、总磷（TP）分析,探讨了TN、TP与TOC的相关性;从而为指导三疣梭子蟹土池育苗产业的可持续发展提供理论依据。对育苗年限不同的7个土池（A、B、C土池池龄7年,D、E、F池池龄3年,G池为空塘,作为空白对照。）营养盐通量的检测得到,Z1期7年期土池NH⁴⁺-N、NO₂--N、NO₃--N、DIN的平均交换通量分别为:196.52、1.18、50.69、248.39μmol·m^-2·d^-1。3年期土池的上述无机氮平均通量分别为:168.55、0.14、15.57、184.26μmol·m^-2·d^-1。育苗年限高的池塘表现出较高的无机氮交换通量。氨氮（NH₄+-N）占了总无机氮（DIN）界面交换通量的大部分,占总无机氮界面交换通量的79.12%～91.47%。活性磷也表现出和无机氮类似的变化规律。随着温度的升高,DIN、H₂PO₄ˉ的交换通量都增高;pH对三疣梭子蟹土池无机氮交换通量的影响表现为中性>碱性>酸性;不同pH条件下磷释放量顺序为酸性>碱性>中性;在厌氧条件下氨氮的界面交换通量大大增加,加入生物抑制剂后DIN的界面交换受到抑制;富氧环境下,活性磷的界面交换通量略大于贫氧环境下的交换通量;在加入氯化汞后,活性磷的交换通量显著增加。2007年5月和2008年5月两个时间点的柱状沉积物间隙水DIN和H₂PO₄ˉ的浓度大体表现为小于10cm时在垂直深度上均呈现明显的降低趋势。由于偶然的机会,发现了才女虫的一种危害途径可能是通过对底质的扰动作用改变营养盐特别是氨氮的含量来影响水质的。由相关性分析得出,育苗池塘底泥中的TN与TOC显著正相关（R∈[0.94,0.99] P<0.05）,TP与TOC负相关,但是显著性不强（R∈[-0.85,-0.46]）更多还原

【Abstract】 This study is designed to analysis the impact of swimming crab laver rearing to the sediment of the pond by simulating field observations and laboratory experiments. We tested the DIN and phosphorous flux of the pond sediment in which the lava of the swimming crab is in its zoea phase and anaphase of M. By lab simulating experiments we also testing how pH Temperature and Eh affecting the sediment-water flux of nutrition. the use of self-designed in our laboratory without disturbance sediment collector collecting sediment samples to detect various types of inorganic nitrogen , reactive phosphorus in the sediment interstitial water of the vertical distribution; through years of different nursery pond sediment soil total organic carbon （TOC）, total nitrogen （TN）, total phosphorus （TP） analysis, discussed the TN, TP and TOC, correlation; so as to guide three trituberculatus nursery industry in earthen ponds provide a theoretical basis for sustainable development.By testing the Z1 phase of 7 Year and 4 Year’s pond, which are different in the rearing age, we now know the flux of NH⁴⁺-N、NO^2--N、NO^3--N、DIN in 7 year’s pond is 196.52、1.18、50.69、248.39μmol·m-2·d-1 respectively. And in 4 year’s pond, it is 168.55、0.14、15.57、184.26μmol·m-2·d-1 .The relatively old age pond is high in DIN flux, NH⁴⁺-N occupied a huge part of the DIN sediment-water flux, about 79.12%～91.47%. The flux of the phosphorous is in the same round.The flux of DIN and H2PO4ˉis increased when the temperature is high. pH can also affected the DIN flux : Neutral>basic>Acid. The flux of NH⁴⁺-N is inceased if there is an anaerobic environment. Flux of DIN is restricted is the Biological inhibitors is added. While the flux of phosphorous is increased once the HgCl is added in.In May 2007 and May 2008 two time points columnar sediment pore water concentrations of DIN and H2PO4ˉgenerally when the performance is less than the vertical depth of 10cm showed a significant reduction. Due to chance, found a talented woman worm may be by way of a hazard of sediment disturbance on the changing role of nutrients, especially nitrogen content to influence the water quality.Derived from the correlation analysis, nursery pond sediment TN and TOC in a significant positive correlation （R∈[0.94,0.99] P <0.05）, TP was negatively correlated with the TOC, but the更多还原