【作者】 郭杨志；

【导师】 周亚滨；

【作者基本信息】 黑龙江中医药大学 ， 中医学， 2011， 硕士

【摘要】 目的：探讨养心颗粒对P53基因转染易损斑块家兔模型血清瘦素(Leptin)含量的影响,研究养心颗粒稳定易损斑块的作用机制。方法：以球囊损伤家兔颈总动脉结合高脂饮食喂养8周,8周末在颈总动脉斑块形成处转染重组人P53腺病毒载体制备易损斑块模型。将模型家兔随机分为模型对照组、养心颗粒组、辛伐他汀组,每组6只,另取6只家兔作为空白对照组；给药2周,10周末药物触发斑块破裂,耳缘静脉取血,用ELISA法检测血清瘦素含量结果：各模型组在8周末血清瘦素含量升高,与空白对照组相比差异具有统计学意义(P<0.05)；10周末,各治疗组血清瘦素含量降低,与模型对照组相比差异具有统计学意义(P<0.05)；10周末,养心颗粒组血清瘦素含量降低,与辛伐他汀组相比差异具有统计学意义(P<0.05)。结论：养心颗粒能明显降低P53基因转染易损斑块家兔模型血清瘦素含量,这可能为其稳定易损斑块的作用机制之一。更多还原

【Abstract】 Objective:To study the effect of Yangxin granule on serum leptin in vulnerable plaque of rabbit model with P53 gene transfection.Method:Carotid artery wall injuries were induced by intravascular balloon in rabbits. At the end of the 8th week, recombinant adenovirus vector for transfecting P53 gene were injected to plaques of carotid segments. Rabbits were randomly divided into four groups:Model control group, Yangxin granule group, simvastatin group and blank group (6 in each group). Following of administration for two weeks, plaques were ruptured because of pharmacological triggering. At the end of the 8th and 10th weekend, the contents of serum leptin was detected by ELISA method.Results:Compared with blank group, the content of serum leptin in other groups were obviously increased at the end of the 8th week(P<0.05).Compared with model control group, the content of serum leptin in treatment groups was declined at the end of 10th week. There was significant difference between Yangxin granule group and simvastatin group (P<0.05).The content of serum leptin in Yangxin granule group was lower than that in simvastatin group(P<0.05).Conclusion:Yangxin granule could remarkably decrease the content of serum leptin in vulnerable plaque of rabbit model with P53 gene transfection, which might be one of the mechanisms for stabilizing vulnerable plaque.更多还原