【作者】 王树娟；

【导师】 段立清；

【作者基本信息】 内蒙古农业大学 ， 农业昆虫与害虫防治， 2011， 硕士

【摘要】 舞毒蛾核型多角体病毒（ Lymantria dispar multiple embedded nucleopolyhedrovirus,LdMNPV）已成为控制舞毒蛾种群动态的重要生物因素之一。荧光素（Tinopal LPW）能够有效的提高LdMNPV对舞毒蛾幼虫的防治效果,寄主植物在利用LdMNPV防治舞毒蛾幼虫的过程中也有重要的作用。本文测定了Tinopal LPW---LdMNPV---寄主植物三者间的相互作用及对亚洲型舞毒蛾的影响。为了更好的利用LdMNPV对亚洲型舞毒蛾幼虫进行生物防治,本文采用食料给毒法测定了不同地理品系LdMNPV对取食青杨的亚洲型舞毒蛾2龄幼虫的致死与亚致死作用。结果表明:LdMNPV-D、LdMNPV-H、LdMNPV-J 3个地理品系的LC₅₀分别为33、39、1076 OBs/μL,LT₅₀分别为10.5、13.5、8.9d;亚致死剂量的LdMNPV 3个地理品系对亚洲型舞毒蛾的发育历期、雌雄性比及蛹重均有一定影响,主要的表现为:发育历期延长、雌雄性比升高及蛹重增加等。为提高LdMNPV防治效果,在室内采用食料给毒法测定了不同浓度的Tinopal LPW对LdMNPV不同地理品系的增效作用及光保护作用,并比较了荧光素对不同病毒品系致死中浓度(LC₅₀)及致死中时间(LT₅₀)的影响。结果表明病毒浓度相同时,LdMNPV 3个地理品系随着Tinopal LPW浓度的增加亚洲型舞毒蛾幼虫的死亡率也增高。1% Tinopal LPW对LdMNPV不同品系均有较强的增效作用,添加1% Tinopal LPW的LdMNPV D、H和J 3个品系对取食青杨的亚洲型舞毒蛾幼虫的致死中浓度(LC₅₀)比不添加1% Tinopal LPW的LC₅₀分别降低了33、24和61倍。此外,1% Tinopal LPW使3个品系的致死中时间(LT₅₀)分别缩短了2.9、5.3、1.2d。Tinopal LPW对LdMNPV D、H和J 3个品系均有光保护作用,添加1% Tinopal LPW后在距离30W紫外灯40cm下照射16h后,它们保持的毒力比未添加Tinopal LPW分别高1.8、2.6和1.8倍。更多还原

【Abstract】 Lymantria dispar multiple embedded nucleopolyhedrovirus has one of the important factors on control the population trends of gypsy moth. The optical brightener can effectively improve the control effect of the LdMNPV to gypsy moth larvae. And the host plants have important role in use LdMNPV to prevent gypsy moth larvae. This study determined the relation of Tinopal LPW---LdMNPV---host plants and the influence to the Asian gypsy moth.In order to use of the LdMNPV to Asian gypsy moth larvae to the biological control better, the study use the diet to poison method to determine the lethal effect and sublethal effect of different geographic strains LdMNPV, which to the 2nd instar Asian gypsy moth larvae feeding poplar （Populus cathayana）. The results showed: The LC50s of LdMNPV-D, LdMNPV-H, LdMNPV-J were 33, 39 and 1076 OBs/μL and the LT50s of the three isolates were 10.5, 13.5 and 8.9d. The sublethal dose of three geography LdMNPV strains have a certain influence to the developmental time, the sex ratio and the pupal weight of Asian gypsy moth larvae. The sublethal dose of LdMNPV-H and LdMNPV-J prolonged the developmental time of Asian gypsy moth, and the total insects stage had significant difference with the contrast; LdMNPV-D also prolonged the developmental time of Asian gypsy moth but the total insects stage had no significant difference with the contrast. The sublethal dose of three geography LdMNPV strains reduced the sex ratio of the females than males. LdMNPV-D strains had significant difference with the pupal weight of female and male ones, but LdMNPV-J strains have highly significant difference with the pupal weight of the female ones. The sublethal dose of LdMNPV-H have no significant effects to the the pupal weight of the female and male ones.In order to select the geographic isolates of gypsy moth multiple embedded nucleopolyhedrovirus （LdMNPV） and enhance their activities, the Asian gypsy moth 1st instar larvae were reared on artificial gypsy moth diet until just before inoculation. The 2nd instar larvae were inoculated using the diet plug method and then were transferred to poplar （Populus cathayana） leaves at laboratory. UV protection function of Tinopal LPW to geographic isolates of LdMNPV and its optimum concentration were tested. The virulence of the three LdMNPV isolates （LdMNPV-D, LdMNPV-H, LdMNPV-J） with and without optical brightener to the 2^nd instar Asian gypsy moth larvae were compared. The results showed out that when the virus concentrations were same but with the different concentration of optical brightener, the mortality of Asian gypsy moth larvae increased as the increasing of Tinopal LPW concentration, the higher the concentration, the higher the mortality. As a viral enhancer, 1% Tinopal LPW increased the virulence of 3 virus isolates to the 2^nd instar Asian gypsy moth larvae. The LC₅₀s of LdMNPV-D, LdMNPV-H, LdMNPV-J with 1% Tinopal LPW were 1.0, 1.6 and 17.6 OBs/μL, respectively. Whereas the LC₅₀s of them without 1%Tinopal LPW were 32.9, 39.0 and 1076.4 OBs/μL, respectively. The virulence of LdMNPV-D, LdMNPV-H, LdMNPV-J were increased 33, 24 and 61 times by adding 1%Tinopal LPW into the virus solution, respectively. In addition, the LT50s of the three isolates with 1%Tinopal LPW were 2.9d, 5.3d and 1.2d shorter than that of the virus without 1%Tinopal LPW. 1%Tinopal LPW had UV protection function to the LdMNPV isolates. After16 hours exposure to a 30W UV lamplight at a distance of 40cm , the original virulence of LdMNPV-D, LdMNPV-H, LdMNPV-J with 1% Tinopal LPW were 1.8, 2.6 and 1.8 times higher than that without 1% Tinopal LPW respectively.更多还原