【作者】 张文佳；

【导师】 韦力；

【作者基本信息】 广西医科大学 ， 人体解剖学， 2011， 硕士

【摘要】 目的:探讨⁶⁰Co-γ射线全脑照射对小鼠视觉传导通路的影响。方法:20只昆明小鼠随机分成2组,每组10只,分别以⁶⁰Co-γ射线剂量0Gy、30Gy的强度进行照射,于照射后1月,各组随机取出7只小鼠,利用荧光金逆行追踪技术以及视网膜铺片技术,逆行追踪观察小鼠视网膜神经节细胞数目;另外各组再取出3只,灌注后制作超薄切片,电镜下观察小鼠外侧膝状体以及视束处的超微结构改变。结果:1、与0Gy组相比,30Gy组视网膜上的荧光金阳性细胞数明显减少（P<0.01）。2、正常组外侧膝状体及视束（opt）处视神经髓鞘完整,轴浆均匀,两层之间存在双层膜结构,轴膜层与层之间排列整齐,轴浆内可见清晰的线粒体、微管和微丝,各正常对照组间无明显差异。而30Gy实验组的髓鞘发生溶解,大多数轴突的轴膜与髓鞘之间出现间隙,可见线粒体肿胀,轴突间出现空泡,轴浆内呈电子低密度,颗粒状变性。结论:大剂量全脑照射可能影响小鼠的视觉传导通路,造成小鼠外侧膝状体和视束超微结构的改变以及视网膜神经节细胞数目的减少。更多还原

【Abstract】 Objective: To observe the changes of visual pathway of mice after ⁶⁰co-γwhole brain ray irradiationMethods: 20 mice were randomly divided into 2 groups: control group and 30Gy-irradiated group （large dose irradiated group）. After one month of irradiation, 7 mice were randomly selected in each group to evaluate the visual pathway changes, with the methods of retinal preparation and fluorogold （FG） retrograde tracing technique. The last 3 mice were selected to make into ultrathin sections.The uhrastructural changes of lateral geniculate nucleus and optic nerve were observed by electronic microscope.Results: 1. compared to the control group , the FG-positive cells in 30Gy group were significantly decreased （P<0.01） in one month after irradiation. 2. The optic nerve of optic tract （opt） in control group at myelin, uniform axoplasm, membrane structure between two layers integrity, the axis between the layers and layers arranged in neat, axonal mitochondria, microtubules and microfilaments were clearly visibled. There is no significant difference in control group. The myelin was dissolved in 30Gy group, the gap emerged between most of the axis of the axon membrane and the myelin sheath, swelled mitochondria was visibled, the axoplasm was electron density, granular degeneration.Conclusion: The visual pathway of mouse maybe change, the uhrastructural changes of lateral geniculate nucleus and optic nerve ,the amount of FG positive cells were observed after large dose whole brain ray irradiation.更多还原