【作者】 商福民；

【导师】 张玉杰；

【作者基本信息】 滨州医学院 ， 皮肤性病学， 2010， 硕士

【摘要】 银屑病是一种慢性炎症性自身免疫性疾病,但其发病机制和治疗仍是世界难题。近几年银屑病与趋化因子及受体关系的研究成为热点。本次研究我们采用ELISA方法检测NB-UVB治疗寻常型银屑病患者前后外周血CC型趋化因子MCP-1及MIP-1α水平的变化,RT-PCR方法检测寻常型银屑病患者外周血单一核细胞CC型趋化因子受体CCR1mRNA的表达。以探讨NB-UVB是否可能通过影响患者外周血某些CC型趋化因子或受体的表达而达到治疗银屑病的目的。目的:检测窄谱中波紫外线(NB-UVB)对银屑病患者外周血CC型趋化因子(MCP-1,MIP-1α)及CC型趋化因子受体1(CCR1)表达水平的影响。探讨NB-UVB是否通过影响银屑病患者外周血MCP-1和MIP-1α及CCR1的表达而发挥作用,为NB-UVB治疗银屑病提供更多的理论依据。方法:采用双抗体酶联免疫吸附法(ELISA)分别检测NB-UVB照射治疗银屑病患者前后外周血CC型趋化因子MCP-1及MIP-1α的值。采用RT-PCR法检测NB-UVB照射治疗患者前后外周血单一核细胞CCR1mRNA表达水平。结果:寻常型银屑病患者治疗前外周血中MCP-1及MIP-1α的值都明显高于正常对照组,照射治疗15次后,患者外周血中MCP-1较治疗前明显降低,差异具有统计学意义(P<0.01)。而MIP-1α与治疗前比较差异无统计学意义(P>0.05)。寻常型银屑病患者治疗前外周血单一核细胞CCR1mRNA表达明显高于正常对照组,照射治疗15次后,外周血单一核细胞CCR1mRNA表达较治疗前明显降低,差异具有统计学意义(P<0.01)。结论:NB-UVB可能通过降低银屑病患者外周血MCP-1及CCR1的表达而发挥作用。更多还原

【Abstract】 Psoriasis is a chronic inflammatory autoimmune disease. However, the pathogenesis and treatment remains the world’s problems. In recent years, the relationship between psoriasis and chemokines (include chemokine receptors) has become popular. In this study, enzyme-linked immunosorbent assay (ELISA) technique was used to detect CC chemokines MCP-1 and MIP-1αvalues in peripheral blood of psoriasis patients before treatment and patients after NB-UVB treatment and healthy controls; Reverse transcription-polymerase chain reaction (RT-PCR) technique was applied to semi-quantitatively analyze CCR1 mRNA expression levels in peripheral blood mononuclear cells (PBMC). In order to investigate whether NB-UVB through regulate the expression of certain CC-type chemokine (or its receptor) to achieve the purpose of anti-psoriasis.Objective: To observe effect of narrow-band ultraviolet B (NB-UVB) phototherapy on peripheral blood CC chemokines (MCP-1, MIP-1α) and CC chemokine receptor 1 (CCR1) in patients with psoriasis. Further clarify whether NB-UVB through influencing the expression of MCP-1 and MIP-1αand CCR1 to achieve the purposes of therapy, provide more theoretical basis for NB-UVB in the treatment of psoriasis.Methods: Serum levels of MCP-1 and MIP-1αwere measured by double-antibody enzyme-linked immunosorbent assay (ELISA) in patients with psoriasis vulgaris before and after the treatment with NB-UVB. CCR1mRNA expression levels in peripheral blood were detected by RT-PCR.Results: Before treatment, MCP-1 and MIP-1αvalues in peripheral blood of patients with psoriasis vulgaris were significantly higher than the normal controls. After 15 times phototherapy, the serum levels of MCP-1 decreased significantly, the difference has statistically significant (P <0.01). While the serum levels of MIP-1αshowed no significant difference before the treatment than after the treatment (P> 0.05). CCR1 levels in peripheral blood mononuclear cells of psoriasis patients before treatment was significantly higher than those in the normal controls. After 15 times phototherapy, CCR1 expression levels in peripheral blood mononuclear cells decreased significantly, the difference has statistically significant (P <0.01).Conclusion: NB-UVB partly through reducing the expression of MCP-1 and CCR1 in peripheral blood of psoriasis to achieve treatment objectives.更多还原