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实时PCR仪荧光信号监测系统的研究
Research on the Fluorescence Signal Monitoring System of Real-time PCR
【作者】 刘娟容；
【导师】 陈章位；
【作者基本信息】 浙江大学 ， 机械电子工程， 2011， 硕士
【摘要】 聚合酶链反应(PCR)是生物医学领域的一项革命性创举,实时PCR技术实现了PCR从定性到定量的飞跃。实时PCR仪已经广泛应用于分子生物学和医学等领域,代表着PCR技术的发展趋势。实时PCR仪荧光信号监测系统是实时PCR仪的一个核心子系统,提供了对荧光信号的数据处理与监测,是定量分析的前提与基础。本文开发实时PCR仪荧光信号监测系统,通过实现对检测装置的运动控制、荧光信号的数据处理实现对荧光信号的监测,本文的主要内容如下：第一章阐述实时PCR系统在国内外的发展概况,阐述本文的研究目标和意义,提出本文的研究内容和方法。第二章介绍实时PCR仪荧光信号监测系统的硬件设计方案,阐述硬件结构的特点及优点,根据硬件设计方案,搭建硬件系统。第三章介绍实时PCR仪荧光信号监测系统的软件开发工具,从运动控制模块及荧光信号数据采集与处理模块两大方面对软件系统进行分析与设计。第四章介绍运动控制模块的实现方法,阐述插补技术的原理及分类,对数据采样插补算法进行优化并应用到本系统中,研究中断控制方法,对检测路径进行规划,最终实现对运动控制模块的控制。第五章介绍荧光信号数据采集与处理模块的实现方法,将多线程技术应用于数据采集与处理模块中,分别从数据段选取、数字滤波、数据平滑、峰值识别、信号归一化五个方面对荧光信号进行数据处理,最终实现对荧光信号的监测,通过仿真与实验相结合检验荧光信号监测系统性能。第六章对全文进行总结,对后续工作进行展望。
【Abstract】 Polymerase chain reaction(PCR) is a revolutionary creation in the biomedical field, real-time PCR technology realizes a leap from qualitative to quantitative. real-time PCR instrument has been widely used in molecular biology and medicine, what’s more, it’s the development trend of PCR technology. As a core subsystem of real-time PCR instrument, fluorescence signal monitoring system is the precondition and foundation for quantitative analysis, it provides fluorescence signal data processing and monitoring.The fluorescence signal monitoring system of real-time PCR instrument has been developed in this paper, which could implement the motion control of detection device, the data processing and the monitoring of fluorescence signal. The following are the main contents of this paper:The first chapter discusses the development of real-time PCP instrument at home and abroad, explains research objectives and research significance, proposes research contents and research methods.The second chapter describes the hardware designing of fluorescence signal monitoring system, and establishes a hardware system according to the hardware designing.The third chapter describes the development tools of fluorescence signal monitoring software system, analyses and designs the software of motion control module and data acquisition and processing module.The forth chapter describes the implementation of motion control module, explains the theory and categories of interpolation algorithm, proposes optimal sampled data interpolation algorithm and applies it to this system, researches interrupt control and fluorescence signal detection path planning, implements the motion control of fluorescence detection system.The fifth chapter describes the implementation of data acquisition and processing module, applies multithreading to this system, processes fluorescence signal include selecting the data segment, digital filter, data smoothing, peak identification, signal normalized, implements monitoring of fluorescence signal, simulates and makes experiment about fluorescence signal monitoring system.The last chapter summarizes this paper and prospectes the following research.