【作者】 曾礼；

【导师】 周朴华；

【作者基本信息】 湖南农业大学 ， 作物， 2010， 硕士

【摘要】 本文调查了邵阳市龙牙百合的生产状况,研究了龙牙百合小鳞茎离体诱导与脱毒培养。生产状况调查结果表明：1、经过长期的无性繁殖种植,由于病毒积累,邵阳市现在种植的龙牙百合种质退化严重,鳞茎球变小、鳞片变短,产量和品质逐步下降,是影响推广种植的主要原因；2、政府扶持力度不够,没有有效组织科技攻关来解决“脱毒”问题；3、龙牙百合的产业链不完全,缺乏深加工,产品档次低,没有形成专业市场；4、部分农民缺技术、缺经验,使龙牙百合种植成本高、风险高,造成龙牙百合的生产不能得到更大面积的推广；5、培养龙牙百合无病毒新种质是恢复优良种性的关键。龙牙百合小鳞茎离体诱导与脱毒培养的研究结果表明：1、利用龙牙百合鳞片诱导产生小鳞茎比较容易；2、小鳞茎的诱导效率与鳞茎的成熟度(收获季节后期成熟度高)、接种鳞片取用部位(中下部)密切相关,培养基6-BA浓度以1.5-2.0mg/L为宜；3、建立起了培养体系。利用诱导的小鳞茎经钝化处理后,切取生长点的部位诱导形成愈伤组织,再形成小鳞茎成苗：4、待小鳞茎生根成株,移入田间种植,进行脱毒鉴定,如成功即是脱毒的简便之法。更多还原

【Abstract】 The production situation of Longya lilium (Lilium brownii F. E. Brown var. viridulum Baker) in Shaoyang district was investigated and studies on bulb in vitro induction as well as detoxification. The main researching results are as follows:The market investigation demonstrates that the current production allows no optimism. To start with, the quality and the quantity of Longya lilium produced in this district are falling down due to the seed degeneration. In addition, the local government fails to offer enough support to deal with seed detoxification. Furthermore, the industrial chain is incomplete, lack of deep processing brings no higher grade products and thus no specialized market. Finally, the planters are at a great risk of high cost but low profit because of their insufficient planting knowledge and experience.The experimental study focused on bulb induction and detoxification culture in vitro gives some progressive suggestions. Firstly, it is somewhat easy to induce little bulbs from the scale of Longya lilium. Secondly, the inducing efficiency is closely related to the scale’s maturity (late period) and the inducing part (middle or below), and the culture 6-BA is 1.5-2.0 mg/L. Thirdly, a culture system is established. Young plants are formed from purified little bulbs with a series of processes. Lastly, the young plants are transplanted, then the detoxification can be appraised.更多还原