【作者】 修暖暖；

【导师】 于俊生；

【作者基本信息】 山东中医药大学 ， 中医内科学， 2010， 硕士

【摘要】 目的：研究和肾络治疗5/6肾切除慢性肾功能衰竭模型大鼠肾组织增殖细胞核抗原(PCNA)及黏附分子-1(ICAM-1)表达的影响,探讨其对肾纤维化的保护作用。方法：采用5/6肾切除模型大鼠,设立正常组、模型组、西药福辛普利组、中药低剂量组、中剂量组及高剂量组。检测各组24 h尿蛋白定量,血清尿素氮(BUN)、血清肌酐(SCr)、总蛋白(TP)、白蛋白(Alb)、甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDI)、高密度脂蛋白(HDL)、血红蛋白(HB)水平,取残肾组织行HE染色,光镜观察肾组织病理变化,免疫组化检测肾增殖细胞核抗原(PCNA)及黏附分子-1(ICAM-1)的变化。结果：中药组与福辛普利治疗组24小时尿蛋白排泄量、BUN.Scr.血脂水平明显低与模型组,中药中剂量与高剂量组提高白蛋白及血红蛋白优于福辛普利组；增殖细胞核抗原与细胞间黏附因子-1术后表达较正常组升高,用药组表达明显低与模型组。结论：1.和肾络可明显减少5/6肾切除大鼠的尿蛋白排泄量、纠正贫血及脂代谢紊乱,降低血肌酐和尿素氮,改善肾功能,改善肾组织的病理损伤,能够保护残存肾单位,延缓肾纤维化的进展。2.抑制PCNA与ICAM-1在肾组织内的过度表达；延缓肾脏纤维化的过程。更多还原

【Abstract】 Objective:To study the expression of proliferating cell nuclearantigen(PCNA) and intercellular adhesion molecule-1 (ICAM-1) changes of 5/6 nephrectomy rats, which to explore the mechanism of renal fibrosis and the mechanism of Heshenluo on renal fibrosis protection. Methods:Using 5/6 nephrectomy rats divided into 5 groups, which are normal group, model group, treated by Fuxinpuli group, treated by Heshenluo Low-dose group, Heshenluo middle-dose group and High-dose group. Urinary protein excretion for 24 hours, blood urea nitrogen(BUN), serum creatinine(Scr), total plasma protein (TP), hemoglobin(Hb), plasma albumin(ALB), triglyceride(TG), cholesterol(CHO), high density lipoprotein(HDL), low density lipoprotein(LDL). Take the renal tissue from the operative side. HE staining and immunohistochemical methods were used to detect the changes of proliferating cell nuclearantigen(PCNA), intercellular adhesion molecule-] (ICAM-1). Results: Compared with the untreated 5/6 nephrectomized group, Urinary protein, BUN, SCr, TG. TC, LDL of Heshenluo groups and Fuxinpuli group were substantially decreased(P< 0.05). Heshenluo middle-dose group and High-dose group was better in increasing ALB and HB than Fuxinpuli group. The expression of proliferating cell nuclearantigen(PCNA), intercellular adhesion molecule-1 (ICAM-1) in 5/6 nephrectomy rats is higher than in normal group. It was less in Heshenluo groups and Fuxipuli group. Conclusion:1 The Heshenluo can decrease the loss of rat’s urine protein and the production of BUN, SCr, TG, TC, LDL, increasing ALB and HB, protect the renal function, improve the pathological lesion of renal tissue, protect the remaining nephron, retard renal fibrosis.2 Inhibit the excessive expression of proliferating cel nuclearantigen(PCNA), intercellular adhesion molecule-1 (ICAM-1), retard renal fibrosis.更多还原