【作者】 王晓蕾；

【导师】 李廷利；

【作者基本信息】 黑龙江中医药大学 ， 中药学， 2010， 硕士

【摘要】 目的:甘麦大枣汤抗焦虑作用及作用机制的相关研究。方法:以小鼠为研究对象,应用高架十字迷宫复制焦虑动物模型,从行为学角度观察性别、体重、测试时间等因素对小鼠高架十字迷宫实验结果的影响;在此基础上进行甘麦大枣汤抗焦虑作用的量效关系和时效关系研究;最后应用高效液相-电化学检测法,测定小鼠海马中5-HT的含量变化,确定甘麦大枣汤抗焦虑作用的作用机制。结果:1.性别对高架十字迷宫实验结果的影响:雌性组与雄性组比较OE%、OT%、总次数不具有显著性统计学差异( P>0.05)。2.体重对高架十字迷宫实验结果的影响:与体重20.0g±1.0g组比较,15.0g±1.0g组OE%具有显著性差异( P<0.05)25.0g±1.0g组OE%具有极显著性差异(P<0.01);15.0g±1.0g组与25.0g±1.0g组OT%均具有极显著性差异(P<0.01);15.0g±1.0g组总次数具有显著性差异(P<0.05)。3.测试时间对高架十字迷宫实验结果的影响:与测试时间14:00-17:00组比较,19:00-21:00组OE%具有极显著性差异(P<0.01);9:00-12:00组OT%具有显著性差异( P<0.05) 19:00-21:00组OT%具有极显著性差异(P<0.01);9:00-12:00组总次数具有极显著性差异(P<0.01)。4.从甘麦大枣汤抗焦虑作用的量效关系研究可知:当给药剂量为10倍时OE%与空白组比较具有极显著性差异(P<0.01),给药剂量为20倍时OE%达到最大值与空白组比较具有极显著性差异( P<0.01),继续增加给药剂量药效逐渐降低;当给药剂量为5倍时OT%与空白组比较具有显著性差异(P<0.05),20倍时OT%达到最大值与空白组比较具有极显著性差异(P<0.01),继续增加给药剂量药效逐渐降低。5.从甘麦大枣汤抗焦虑作用的时效关系研究可知:给药3天时与本组空白组比较,OE%具有显著性差异(P<0.05),给药7天OE%达到最大值与本组空白组比较具有极显著性差异(P<0.01),继续增加给药天数药效逐渐降低;给药5天时与本组空白组比较OT%具有显著性差异(P<0.05),给药7天OT%达到最大值与本组空白组比较具有极显著性差异(P<0.01),继续增加给药天数药效逐渐降低。6.从甘麦大枣汤抗焦虑作用的作用机制研究可知:甘麦大枣汤能降低小鼠海马内5-HT的含量,与空白组比较具有极显著性差异(P<0.01)。结论:1.性别对高架十字迷宫实验结果的影响没有显著性统计学差异。2.体重对高架十字迷宫实验结果有一定影响,体重为20.0g±1.0g的小鼠焦虑状态最明显。3.测试时间对高架十字迷宫实验结果有一定影响,测试时间为14:00-17:00时小鼠焦虑状态最明显。4.甘麦大枣汤抗焦虑作用呈现一定的量效关系和时效关系变化,但是不呈现剂量依赖性。最佳给药剂量为20倍剂量;最佳给药时长为连续给药7天。5.甘麦大枣汤可能是通过降低小鼠脑内海马区5-HT的含量发挥抗焦虑作用。更多还原

【Abstract】 Objective: Studies on the anxiolytic effect and function mechanism of Ganmaidazao Tang.Methods: Take the mice as the object of study, application EPM duplication anxious animal model, from behavior study angle the anti-anxious function. From behavior study angle factors and so on observation sex, weighe, testing time to EPM experiment result influence; Based on this research about dose-response relationship and time-concentration of the Ganmaidazao tang anti-anxious; Used HPLC-EC technique, in determination the region of hippcampus mice 5-HT content change, determined Ganmaidazao Tang affects anxious function mechanism.Results:1. Sex to experimental result influence:female group and male group OE%,OT% and locomotor activity P>0.05.2. Weight to experimental result influence: compares with the weight for 20.0±1.0g group, the weight for 15.0g±1.0g group OE% P<0.05, 25.0g±1.0g group OE%P<0.01;15.0g±1.0g and 25.0g±1.0g group OT%P<0.01; 15.0g±1.0g group locomotor activity P<0.05.3. Test time to experimental result influence:compares with the 14:00-17:00 group, 19:00-21:00 group OE% P<0.05; 9:00-12:00 group OT% P<0.05, 19:00-21:00 group OT% P<0.01; 9:00-12:00 group locomotor activity P<0.01.4. The experimental results of the dose-response relationship showed that from the doses of 7.0g/kg started work there was significant difference OE% P<0.01; the doses of 14.g/kg reached the climax, there was extremely significant difference between the OE% and OT%; the doses of 3.5g/kg OT% P<0.05.5 . The experimental results of the time-response relationship showed: the experimental results of the time- concentration relationship showed that giving medicine for three days started work, OE% P<0.05; giving medicine for seven days reach the climax, there was extremely significant different between OE% and OT% P<0.01; giving medicine for five days OT% P<0.05.6. The experimental results of function mechanism of Ganmaidazao Tang, it can reduce content 5-HT of the region of hippocampus mice P<0.01.Conclusion:1. The sex hasn’t affect EPM results.2. The weight affects EPM results: weight of 19.0g-21.0g is better.3. Test time affects EPM results: test time of 14:00-17:00 is better.4. Ganmaidazao Tang could improve the OE% and OT%,14.0g/kg is choosed for the best doses, seven days is choosed for the best time.5. Ganmaidazao Tang can reduce content 5-HT of the region of hippcampus mice更多还原