【摘要】 利用38对SSR引物对来源于不同国家与地区的171份野生和栽培新麦草单株种质进行遗传多样性和群体结构分析。研究结果显示,有效引物在新麦草单株材料间共获得308个位点,多态性位点275个,多态性百分率为89.29%,供试新麦草材料的遗传相似性系数介于0.537～0.899之间,平均为0.742。群体结构分析、主成分分析与NJ聚类法均将供试材料分为3个类群,且类群间的材料相互渗透形成混合类群,分类结果与遗传相似性系数分析表明171份新麦草单株种质分布较集中;3种分类方法对新麦草材料3个不同类群的划分结果基本一致,但混合类群的划分存在差异,每个类群的新麦草单株材料来源背景不同,未表现出明确的地域性规律。更多还原

【Abstract】 Genetic diversity and population structure analysis of 171 wild and cultivated PsathyrostachysNevski individual germplasms from different countries and regions were conducted by 38 pairs of SSR primers.The result by using the primers revealed a total of 308 loci among Psathyrostachys Nevski individuals,with 275 polymorphic loci and a polymorphism percentage of 89.29%.The genetic similarity coefficient of the tested materials ranged from 0.537 to 0.899 with an average of 0.742.These accessions were divided into three groups by population structure analysis,principal component analysis and NJ clustering.The genetic similarity coefficient analysis showed that the distribution of Psathyrostachys Nevski individuals was more concentrated.The classification using three methods generally revealed three different groups of Psathyrostachys Nevski materials,despite that there were slightly different in the divisions of mixed groups.Since these Psathyrostachys Nevski individuals in each group showed different on genetic background,no clear correlation between geographic distribution and genetic diversity was observed.更多还原