【摘要】 通过比较固定酶底物法与多管发酵法的检测效果,为监测站耐热大肠菌群的检测方法选择提供技术支持。2018年11月～2019年3月,选取广州市主要饮用水水源地和水源调查点采集的地表水为样本,用固定酶底物法与多管发酵法两种方法分别对51份水样中耐热大肠菌群进行比对试验检测。结果表明:固定酶底物法与多管发酵法相比,在检测一致性方面无统计学上的显著性差异,但固定酶底物法具有检测时间短、效率高、人力成本较低、结果更加精确、安全性较好等优势,既可作为地表水常规检测方法,又可用作应急、灾害时监测的快速筛查方法。更多还原

【Abstract】 Comparing detection results of immobilized enzyme substrate method and multiple tube fermentation technique could provide technical support for the selection of heat-resistant coliform detection methods in monitoring stations. The surface water samples were collected from different drinking water reservoir and water source sites during November 2018 to March 2019. 51 samples were carried to test the existence of thermotolerant coliform bacteria by immobilized enzyme substrate method and multiple tube fermentation technique. Comparing the results of large number of parallel water samples which were tested by the above two methods, results showed that there was no statistically significant difference. However, immobilized enzyme substrate method has some advances, such as the shorter detection time, the higher efficiency, the lower labor cost and the safer performance, particularly, the results could be more precise. It could be used as a rapid screening method for routine surface water detection or emergency and disaster monitoring.更多还原