【摘要】 【目的】对银耳进行转录组测序分析,分析多糖生物合成途径和挖掘关键功能基因。【方法】采用Illumina Hiseq 2500测序平台对银耳菌丝体进行测序,组装得到unigene序列,并与公共数据库进行对比分析和注释,挖掘果糖和甘露糖代谢通路相关基因。【结果】本研究共得到4.72 Gb raw data,拼接后获得17 008条unigene序列,N50为2073 bp;注释结果表明,76.47%序列能够注释到Uniprot公共数据库,注释到GO分类和KEGG通路的unigene分别为10 152和5671条。进一步分析银耳果糖和甘露糖代谢途径相关基因,挖掘得到74条unigene;其中编码L-iditol 2-dehydrogenase的unigene最多,其次是butanol dehydrogenase。【结论】基于转录组测序数据解析及预测,为研究银耳多糖和其它产物的生物合成途径及分子机制提供了数据支持,也为银耳品质的形成提供理论依据。更多还原

【Abstract】 【Objective】The study aimed to study on the expression of genes involved in the biosynthesis of fructose and mannose metabolism and explore the functional genes in Tremella fuciformis, the transcriptome was sequenced. 【Methods】 By Illumina Hiseq 2500 platform sequencing technology, the transcriptome of the mycelium was sequenced, assembled and annotated. Moreover, unigene related to the fructose and mannose metabolism pathway were identified.【Results】4.27 Gb raw data was generated, and 17 008 unigenes were obtained after splicing, which the N50 was 2073 bp. 76.47 % unigene was annotated in the Uniprot database, which 10 152 and 5671 unigenes were annotated in the GO classification and KEGG pathways. The fructose and mannose metabolism pathway in Tremella fuciformis involved in 74 unigenes, especially unigene coding L-iditol 2-dehydrogenase and butanol dehydrogenase with the most unigenes. 【Conclusion】This study would be established for revealing the biosynthesis mechanism of polysaccharide and other production. At the same time, this study will provide a theoretical basis for quality formation of Tremella fuciformis.更多还原