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单壁碳纳米管对太平洋牡蛎(Crassostrea gigas)的毒性效应及生物体防御机制研究
Study on Toxicity of Single-Walled Carbon Nanotubes (SWCNTs) to Pacific Oyster (Crassostrea gigas) and the Defense Mechanism Involved
【摘要】 碳纳米管的生态安全和健康风险日益受到人们的广泛关注。本文采用典型的海洋底栖生物——太平洋牡蛎(Crassostrea gigas, C. gigas)作为受试生物,研究了单壁碳纳米管(Single-Walled Carbon Nanotubes, SWCNTs)暴露对其造成的毒性效应及牡蛎自身的防御机制,以期为碳纳米管的海洋生态风险评价提供科学依据。在0.1~10 mg·L-1的SWCNTs暴露96 h后,太平洋牡蛎鳃和消化腺中的丙二醛(malondialdehyde, MDA)含量显著增加(P≤0.05),总超氧化物歧化酶(superoxide dismutase, SOD)和过氧化氢酶(catalase, CAT)活性呈现显著的剂量依赖性升高(P≤0.05),cat、hsp70、aox及caspase-7等基因的相对表达量显著上调(P≤0.05)。相比于单独暴露,P-gp蛋白抑制剂Tariquidar与SWCNTs的复合暴露显著增加了鳃和消化腺中MDA含量,产生了更严重的氧化损伤。这些结果表明,SWCNTs暴露对太平洋牡蛎的鳃和消化腺造成了一定程度的氧化损伤,而牡蛎体内的抗氧化系统和多外源性物质抗性机制在防御SWCNTs的过程中起到了至关重要的作用。
【Abstract】 The ecological and health risks of carbon nanotubes are receiving more attention. In this study, Pacific oyster(Crassostrea gigas), a kind of typical marine benthic organism, was exposed to single-walled carbon nanotubes(SWCNTs) to investigate the toxicity induced by SWCNTs and the defense mechanism of C.gigas. After 96-h exposure to 0.1-10 mg L-1 SWCNTs, the content of malondialdehyde(MDA) in gills and digestive glands increased significantly(P≤0.05), the activities of total superoxide dismutase(SOD) and catalase(CAT) showed the significant dose-dependent increase(P≤0.05), and the relative expressions of genes, i.e., cat, hsp70, aox and caspase-7, were significantly up-regulated(P≤0.05). The co-exposure of Tariquidar(P-gp protein inhibitor) and SWCNTs induced the significant increase of the MDA content in gills and digestive glands(P≤0.05), indicating that more serious oxidative damage were caused by the mixture. The results revealed that the antioxidant system and Multi-xenobiotic Resistance Mechanism played vital roles in the defense system of Pacific oyster against the SWCNTs exposure.
【Key words】 SWCNTs; Crassostrea gigas; toxicity; the defense mechanism;
- 【文献出处】 生态毒理学报 ,Asian Journal of Ecotoxicology , 编辑部邮箱 ,2019年01期
- 【分类号】X171.5
- 【被引频次】1
- 【下载频次】74