【摘要】 以铁皮石斛幼嫩的带节茎段为外植体,通过研究植物生长调节剂对类原球茎诱导、增殖和分化的影响,筛选出适宜铁皮石斛类原球茎植株高效再生的条件。结果表明:类原球茎诱导的最适培养基为MS+6-BA 1.0 mg/L+IBA 1.0 mg/L+土豆泥60 g/L+活性炭0.5 g/L+蔗糖25 g/L+琼脂6.5 g/L,茎段接种45 d后,类原球茎诱导率为97.78%;类原球茎增殖和分化的最适培养基为MS+6-BA 2.5 mg/L+IBA 1.0 mg/L,培养30 d,增殖倍数为7.94,培养至60 d,丛生芽诱导率为97.33%;再生苗在MS+NAA 0.5 mg/L培养基中培养30 d,生根率达到100%。更多还原

【Abstract】 In order to establish an efficient plant regeneration system,young stem segments of Dendrobium officinale Kimura et Migo were used as explants,and the effects of plant growth regulators on the protocorm-like body(PLB) induction,proliferation and differentiation were studied. The results showed that the optimum medium for PLB induction was MS + 6-BA 1.0 mg/L + IBA 1.0 mg/L + mashed potato 60 g/L + acticarbon 0.5 g/L + sucrose 25 g/L + agar 6.5 g/L,and the PLB induction rate was 97.78% after cultured for 45 d. The optimum medium for PLB proliferation and differentiation was MS + 6-BA 2.5 mg/L + IBA 1.0 mg/L,the proliferation multiple of PLB reached 7.94 after cultured for 30 d,and the induction rate of cluster bud was 97.33% after cultured for 60 d. The optimum rooting medium was MS + NAA 0.5 mg/L,and the rooting rate was 100% after cultured for 30 d.更多还原