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一株高效降解玉米赤霉烯酮的耐酸耐高温枯草芽孢杆菌的研究
Study on Heat Resistant Bacillus subtilis With High Degradation Efficiency of Zearalenone
【摘要】 为寻求更高效、实用的生物脱毒制剂,提高其应用范围,通过采集酸性、高温的温泉环境样品,筛选获得1株高效降解玉米赤霉烯酮(ZEN)的细菌菌株Y-33,并对该菌株生长和降解特性进行分析。结果表明,菌株Y-33在50℃、pH值5.0条件下孵育36 h,菌液对2μg·mL-1 ZEN的降解率高达93.79%,对20μg·mL-1 ZEN的降解率也达82.40%。从形态、生理生化特性及16S rDNA序列对菌株Y-33进行分析,初步鉴定为枯草芽孢杆菌(Bacillus subtilis)。该菌株发酵上清液在28℃条件下对ZEN降解率为62.02%。金属离子Mn2+能明显增强菌株Y-33发酵上清液对ZEN的降解能力,降解率达81.17%,而Zn2+严重抑制了Y-33发酵上清液对ZEN的降解能力,降解率仅为5.42%。本研究结果为ZEN解毒菌剂的开发与应用奠定了理论基础。
【Abstract】 To seek more efficient and practical biological detoxification strain to improve the application scope, we collected samples from acid and high temperature environment. One strain of bacteria named Y-33, was isolated for efficient detoxification zearalenone(ZEN),. Y-33 strain was incubated at 50℃ and pH value 5.0 for 36 hours, after which the degradation rate of bacterial solution to 2 μg·mL-1 ZEN was as high as 93.79%, and up to 82.40% to 20 μg·mL-1 ZEN. The strain Y-33 was initially identified as Bacillus subtilis with the analysis of its morphological, physiological and biochemical characteristics and 16 S rDNA sequences. The degradation rate in fermentation supernatant of the strain′s was 62.02% at 28℃. Metal ion Mn2+ could significanty enhance the degradation ability of strain Y-33 culture supernatant to ZEN with the degradation rate being 81.17%, while Zn2+ severely inhibited the degradation capability of ZEN by Y-33 culture supernatant to only 5.42%. The results of this study lay the theoretical foundation for the development and application of ZEN detoxifying microbes.
【Key words】 zearalenone; degradation; Bacillus subtilis; aciduric and heat-resistant;
- 【文献出处】 核农学报 ,Journal of Nuclear Agricultural Sciences , 编辑部邮箱 ,2019年07期
- 【分类号】S816.3;TS201.3
- 【网络出版时间】2019-05-14 11:01
- 【被引频次】11
- 【下载频次】423