【摘要】 以S^2-的氧化率为主要考察指标,对从北京黑臭水体东沙河筛选获得的3株高效S^2-氧化土著微生物sp1（Citrobacter sp.）、 sp2（Ochrobactrum sp.）和sp3（Stenotrophomonas sp.）进行复配,获得硫氧化复合菌（sulphur oxidizing composite microorganisms, SOCM）,比较单株菌sp1、sp2和sp3和SOCM对S^2-的氧化效果。结果表明,SOCM对S^2-的氧化能力明显优于单菌株。SOCM在复配比例为1∶1∶1,温度为25℃,初始pH为7时对北京市东沙河黑臭水样中S^2-的氧化效果最好,氧化率最高达到76.7%;同时,色度、COD、NH3-N和TP的去除率可分别达到83.3%、69.2%、77.9%和68.2%。此外,建立了SOCM氧化S^2-的动力学方程。当SOCM初始菌浓度从0.01 g·L^-1逐渐提高到10 g·L^-1时,底物比氧化速率常数Km随之减小,S^2-平均氧化速率提高。但是,当SOCM初始菌浓度从10 g·L^-1逐渐提高到50 g·L^-1时,S^2-平均氧化速率不再随着初始菌浓度的升高而加快。最后,将SOCM接种于北京清河和景观沟渠黑臭水样中,其对S^2-氧化率分别达到67.0%和64.1%;同时,色度亦分别下降了83.3%和79.2%。研究为黑臭水体的微生物法治理提供了参考。更多还原

【Abstract】 In this study, S^2-oxidation ratios was taken as the main index, three indigenous pure strains of sp1（Citrobacter sp.）, sp2（Ochrobactrum sp.） and sp3（Stenotrophomonas sp.） with high efficiently S^2-oxidation were isolated from Dongsha river, a black-stinking water body in Beijing. They were mixed to produce the sulfuroxidizing composite microorganisms（SOCM）.The S^2-oxidation efficiencies of sp1, sp2, sp3 and SOCM were compared. The results showed that SOCM had an obviously better performance on S^2-oxidation than these three pure bacteria, and the best S^2-oxidation effect in the black-stinking water samples from Dongsha river in Beijing occurred at the complex ratio for SOCM production 1∶1∶1, 25 ℃ and the initial pH of 7, and the highest S^2-oxidation efficiency reached 76.7%, and the removal efficiencies of chroma, COD, NH3-N and TP were 83.3%,69.2%, 77.9% and 68.2%, respectively. In addition, the kinetic equation for S^2-oxidation by SOCM was determined. When the initial bacterial concentration of SOCM increased from 0.01 g·L^-1 to 10 g·L^-1, the substrate specific oxidation rate constant Kmdecreased, and the average S^2-oxidation rate increased. When the initial bacterial concentration of SOCM increased from 10 g·L^-1 to 50 g·L^-1, the average S^2-oxidation ratio did not increase any more. Finally, after inoculating SOCM into the black-stinking water samples from Qinghe river and Jingguangouqu river in Beijing, the S^2-oxidation efficiencies reached 67.0% and 64.1%, and their chroma values decreased by 83.3% and 79.2%, respectively. This study provided a reference for the microbial remediation of black-stinking water body.更多还原