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植物乳杆菌素BM-1对大肠杆菌抑菌活性的研究

Bacteriostatic activity of plantaricin BM-1 against Escherichia coli

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【作者】 张含薇谢远红金君华张红星

【Author】 ZHANG Hanwei;XIE Yuanhong;JIN Junhua;ZHANG Hongxing;College of Food Science and Engineering,Beijing University of Agriculture/Beijing Laboratory of Food Quality and Safety/Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products;

【通讯作者】 张红星;

【机构】 北京农学院食品科学与工程学院/食品质量与安全北京实验室/农产品有害微生物及农残安全检测与控制北京市重点实验室

【摘要】 【目的】探究植物乳杆菌素BM-1对大肠杆菌的抑菌活性。【方法】通过管碟法检测植物乳杆菌素BM-1对12株大肠杆菌的抑菌活性,选取不同敏感型大肠杆菌5株,对大肠杆菌生长曲线、活菌数、生物膜的形成进行测定,并采用实时荧光定量PCR方法测定大肠杆菌中细菌素受体基因的表达。【结果】生长曲线、活菌数、生物膜的形成试验结果显示,大肠杆菌CGMCC 1.8723对植物乳杆菌素BM-1敏感性最强;实时荧光定量PCR显示植物乳杆菌素BM-1显著影响大肠杆菌甘露糖磷酸转移酶系统编码基因的表达。【结论】植物乳杆菌素BM-1对大肠杆菌表现出不同水平的抑菌活性。

【Abstract】 【Objective】In this study the bacteriostatic activity ofplantaricin BM-1 against Escherichia coli was investigated.【Method】The bacteriostatic activity of plantaricin BM-1 on Escherichia coli was detected by Oxford Cup bacteriostatic method.Five Escherichia coli strains were selected from different sensitive to plantaricin BM-1,and their growth curves,viable counts,biofilm formation and real-time PCR were determined.【Result】Growth curve,viable count and biofilm formation showed that CGMCC 1.8723 was the most sensitive strain to plantaricin BM-1.Real-time PCR showed that plantaricin BM-1 stimulated the receptor gene expression.【Conclusion】Plantaricin BM-1 exhibited different levels of antibacterial activity against Escherichia coli.

【基金】 北京市教委科研计划项目(KM201810020016)
  • 【文献出处】 北京农学院学报 ,Journal of Beijing University of Agriculture , 编辑部邮箱 ,2019年03期
  • 【分类号】TS201.3
  • 【网络出版时间】2019-03-22 16:34
  • 【被引频次】1
  • 【下载频次】390
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