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两种赤潮藻对汞富集和甲基化影响的研究
Study on the Effect of Two Red-Tide Algae on Mercury Biocondensation and Methylation
【摘要】 研究塔玛亚历山大藻(Alexandriumtamarens)和锥状斯式藻(Scrippsiellatrochoidea)两种赤潮藻对海水中汞的富集和甲基化的影响,探讨赤潮藻对硫铁还原地杆菌GeobactersulfurreducensPCA (G. sulfurreducens PCA)汞生物甲基化的抑制作用。实验结果表明,不同种类藻对汞的耐受性不同,高浓度HgCl2 (≥25μg/L)抑制锥状斯式藻的生长,而对塔玛亚历山大藻的影响较小。两种藻均可有效地富集无机汞,但直接进行汞甲基化的效果不显著。FTIR分析发现,藻细胞表面分泌的大量羧基、氨基和羟基等官能团是富集汞的主要位点。汞-藻-菌实验中,当HgCl2初始浓度为10ug/L时,G. sulfurreducensPCA驱动的汞生物甲基化效率可达(6.38±0.4)%,在G. sulfurreducensPCA与塔玛亚历山大藻共存的实验组中,汞甲基化效率为(1.04±0.44)%,G. sulfurreducensPCA与锥状斯式藻的实验组中汞甲基化效率低至(0.76±0.05)%,两种赤潮藻的加入抑制了G.sulfurreducens PCA的汞生物甲基化。
【Abstract】 The effect of two red-tide algae of Alexandrium tamarens and Scrippsiella trochoidea on mercury(Hg)adsorption and methylation were investigated. The inhibitory effect of two algae on biomethylation of Geobacter Sulfurreducens(G. sulfurreducens) PCA were demonstrated. The growth of Scrippsiella trochoidea was inhibited under exposure to high concentration of HgCl2(≥25 μg/L), but less affecting Alexandrium tamarens. Significant adsorption of Hg2+ was observed onto algal cells, whilst negligible amount of methylmercury(MeHg) was produced by two algae directly. FTIR spectra revealed that hydroxyl, carboxyl and amino groups were major binding sites for Hg2+ adsorption. In Hg-algae-bacteria tests, at initial HgCl2 concentration of 10 μg/L, a maximum mercury methylation efficiency of(6.38±0.4)% was obtained by pure G. sulfurreducens PCA culture as a control,but the efficiency reduced to(1.04±0.44)% with G. sulfurreducens PCA and Alexandrium tamarens coexisting, and a much lower efficiency of(0.76±0.05)% was detected with G. sulfurreducens PCA and Scrippsiella trochoidea coexisting. These results suggested that two red-tide algae inhibited mercury biomethylation of G. sulfurreducens PCA.
【Key words】 mercury; methylation; Scrippsiella trochoidea; Alexandrium tamarens; Geobactersul furreducens PCA;
- 【文献出处】 北京大学学报(自然科学版) ,Acta Scientiarum Naturalium Universitatis Pekinensis , 编辑部邮箱 ,2019年04期
- 【分类号】X55;X17
- 【网络出版时间】2019-06-06 13:35
- 【被引频次】3
- 【下载频次】242