【摘要】 采用生物信息学的方法分析已经在GenBank上注册十字花科植物的DFR基因序列及相应氨基酸序列,并对其理化性质、结构特征、系统进化关系等进行预测。结果表明,十字花科植物的DFR蛋白大部分都有6个外显子,分子质量36 481.89～43 129.21 Da,大多数蛋白亚细胞定位于叶绿体中;除了BnaDFRA402,其他的DFR蛋白均具有酶活性部位、NADP结合位点和底物特异结合位点,这些保守区域形成了5个motifs;每个DFR蛋白均有多个磷酸化位点,以Ser为主,以Thr和Tyr磷酸化为辅;二级结构均由α-螺旋、β-转角、延伸链和无规则卷曲组成,其中α-螺旋为主要结构元件。更多还原

【Abstract】 The DFR gene sequences and corresponding amino acid sequences registered on GenBank in Cruciferae plants were analyzed by means of bioinformatics, their physical and chemical properties,structural characteristics and phylogenetic relationships were predicted. The results showed that most of DFR proteins in Cruciferae plants had six exons with molecular weight ranging from 36 481.89 Da to 43 129.21 Da.Most of the protein subcells were located in chloroplasts. Except BnaDFRA402, other DFR proteins had enzymatic activity sites, NADP binding sites and substrate specific binding sites, which formed five motifs. Each DFR protein had multiple phosphorylation sites,in which Ser was the dominant and Thr and Tyr were the minor. The secondary structures were composed of alpha helix, beta turn, extended strand and random coil, and alpha helix was the main structural element.更多还原