【作者】 曾桂凤；

【导师】 梁鑫淼； 刘建勋；

【作者基本信息】 中国科学院研究生院（大连化学物理研究所） ， 分析化学， 2006， 博士

【摘要】 近年来中药研究正在药学界蓬勃兴起。中药复方是中医用药的主要形式,而配伍理论是中药复方的根本。本论文针对中药配伍中的问题,对复方丹参方剂配伍的化学成分变化、药理药效变化及其药效物质基础进行了研究。论文第二章建立了一套稳定、可靠、重现性好、统一的HPLC方法,分析了丹参、三七及复方丹参配伍前后化学成分变化研究。结果表明,复方丹参48个峰中有24个峰来源于丹参,10个峰来源于三七,还有14个峰是共流出峰,但没有新峰产生;与单味药丹参、三七比较,配伍后有19个峰含量增加,6个峰含量降低。论文第三章考察了丹参、三七按不同比例配伍;丹参、三七用系统溶剂萃取法得到的不同极性组分以及丹参、三七的有效组分按不同比例配伍后的药效变化。结果表明,不同配伍比例药效不同,不同组分药效不同,而以有效组分进行配伍后,有效比例比以生药丹参、三七进行配伍的有效比例要广。为了进一步揭示复方丹参作用的物质基础,论文第四、第五章分别对丹参、三七进行了HPLC-MS/MS分析,在质谱电离和质谱裂解规律的基础上,鉴定了丹参乙酸乙酯组分中28种水溶性成分,氯仿组分中24种脂溶性成分和三七中以皂甙为主的59种成分。最后,在质谱分析的基础上,对丹参水溶性成分的液相色谱保留参数和峰形参数规律进行了初步研究,发现保留参数规律与化合物结构有关,峰形参数规律与化合物的解离有关。更多还原

【Abstract】 In the recent years, the research focus on Chinese Traditional Medicine (TCM) is becoming more and more popular in medicine field. Prescriptions are the main form of TCM. Theory of compatibility is the essential part of TCM. In this paper, the changes of chemical components, the changes of pharmacological activities, and the effective compounds in Fufang Danshen were studied.A stable, reliable, uniform HPLC analytical method with good reproducibility was developed in the second chapter to study the changes of chemical ingredients between single herbs of Salvia miltiorrhizae (SM), Panax notoginseng (PN) and their prescription-Fufang Danshen (FFDS). The results indicated that in the 48 peaks of FFDS, there are 24 peaks contributed from SM,10 peaks contributed from PN, and 14 peaks co-contributed from SM and PN, but no new peaks produced. Comparing FFDS with single herbs of SM and PN, the areas of 19 peaks are increased; while the areas of 6 peaks are decreased.The influence of different compatible ratios of SM to PN in FFDS, different fractions of SM and PN extracted with different solvent, different compatible ratios of effective fractions of SM to effective fractions of PN in FFDS on pharmacological activity were investigated in the third chapter. The results indicated that different ratios of SM to PN in FFDS have different pharmacological activity; different fractions also have different pharmacological activity; and the effective ratios of effectual fractions of SM to effectual fractions of PN are wider than those of SM to PN.更多还原