【作者】 岳辉；

【导师】 毕银丽；

【作者基本信息】 中国矿业大学（北京） ， 环境科学， 2013， 博士

【摘要】 为了研究丛枝菌根真菌在修复植物受损根系中的机理,分析丛枝菌根真菌在野外大田条件下微生物复垦的生态效应,本文通过在室内设计量化伤根试验和在野外大田对接菌紫穗槐动态监测分析相结合的方法对其进行了详细研究。首先,在参阅大量国内外文献的基础上,分析了菌根修复受损根系的研究现状,讨论了丛枝菌根真菌在矿区生态修复监测中存在的实际问题和应用前景。针对采煤沉陷区植物根系存在机械拉伤的实际情况,提出基质内和基质外两种模拟伤根方式并利用五室培养法研究丛枝菌根真菌修复植物受损根系的机理；结果表明菌根真菌能够减缓根系损伤对菌根-植物共生体的负面影响。其次,在野外大田条件下对紫穗槐接种菌根真菌进行了微生物复垦实地应用,系统分析了2009-2012年间接菌和对照区紫穗槐的地上生物量、地下菌丝网络和土壤理化性状的变化规律；利用遥感数据与实地土壤和植物样品采集分析相结合的方法动态监测了微生物复垦的生态效应；引入主成分分析法对微生物复垦的生态效果进行了评价,结果表明接菌有利于矿区植被的恢复和生态重建。最后,对丛枝菌根真菌未来的研究方向，提出了参考建议。更多还原

【Abstract】 In order to study the mechanism of arbuscular mycorrhizal fungi in repair of damaged plant root system, analyse the microbial reclamation ecological effect of arbuscular mycorrhizal fungi in the open field conditions, this paper through the indoor design quantitative root damage experiment and in the open field vaccinating on amorpha fruticosa various dynamic monitoring analysis method carries out combined detail study. Firstly, on the basis of referring to extensive foreign and domestic literature, analysis present research situation of arbuscular mycorrhizal repair damaged root, summarizes the ecological and physiological processes and characteristics, and discusses the the problems and application prospects in the mining area ecological restoration. According to the coal mining subsidence area plant roots exist mechanical strain of the actual circumstance, design the in/out matrix root damage mode test for arbuscular mycorrhizal fungi on plant root repair damaged mechanism research; the results show that mycorrhizal fungi can reduce the negative influence on mycorrhizal-plant symbionts. Secondly, in the open field conditions for various inoculation mycorrhizal fungi on the microbial reclamation field application, the system analyzed in the long period in2009-2012; and then bacteria and control area of various aboveground biomass, underground hypha network and physicochemical properties of soil changes in law, using remote sensing data and the field soil and plant sample collection and analysis method of combining the dynamic analysis of the microbial reclamation ecological effect, the introduction of principal component analysis to microbial reclamation ecological effect was evaluated, the results show vaccinating arbuscular mycorrhizal fungi is helpful to the vegetation restoration and ecological reconstruction in mining area. Finally, some suggestions are presented on the future direction of the arbuscular mycorrhizal fungi research.更多还原