【作者】 闫东；

【导师】 李冀；

【作者基本信息】 黑龙江中医药大学 ， 方剂学， 2012， 博士

【摘要】 目的采用乌头碱、氯化钙两种方法分别诱发大鼠快速性心律失常模型,研究藤黄科金丝桃属植物乌腺金丝桃正丁醇萃取部位抗快速型心律失常作用及其物质基础,并通过观察分离的单体物质对心肌细胞膜Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶的影响探讨乌腺金丝桃发挥抗快速型心律失常作用的机理。方法一、运用Medlab生物信号采集处理系统,通过计算机自动采样分析记录心电图,研究乌腺金丝桃不同提取部位对乌头碱、氯化钙两种方法所诱发的实验性心律失常的作用效果。二、基于离体心脏灌流技术、色谱分离法和波谱鉴定法等技术,分离出乌腺金丝桃抗心律失常有效部位中的化合物三、采用无机磷比色法检测乌腺金丝桃抗快速型心律失常有效部位的化合物对大鼠心肌细胞膜Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶活力的影响；结果一、乌腺金丝桃正丁醇萃取物能够延长乌头碱、氯化钙诱发的大鼠快速型心律失常的出现时间,缩短心律失常持续时间,与模型组比较差异具有统计学意义, P<0.05。二、从乌腺金丝桃正丁醇萃取物中,分离和鉴定了芦丁和金丝桃苷2种化合物。三、金丝桃苷能够提高快速型心律失常大鼠心肌细胞膜Na+-K+-ATP酶活力及Ca2+-Mg2+-ATP酶活力,与空白对照组比较,差异具有统计学意义, P<0.05。结论一、乌腺金丝桃对乌头碱、氯化钙两种方法诱发的快速型心律失常动物模型均有明显的拮抗作用,表明乌腺金丝桃具有较好的抗快速型心律失常作用。二、芦丁和金丝桃苷是乌腺金丝桃抗快速心律失常的有效成分。其中金丝桃苷作用明显,被确定为乌腺金丝桃抗快速心律失常的物质基础之一。三、从乌腺金丝桃中分离的有效化合物金丝桃苷能够提高快速型心律失常大鼠心肌细胞膜的Na+-K+-ATP酶及Ca2+-Mg2+-ATP酶的活力,表明乌腺金丝桃抗快速型心律失常的作用机制可能与调节心肌细胞膜Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶活力有关。芦丁无以上药理作用,其组分抗快速性心律失常的作用可能是通过其他途径实现的。更多还原

【Abstract】 Objective:With the rat arrhythmia modelinduced by two methods of aconitineand calcium chloride, researching Hypericum attenuatum Choisy butanol extract belonged to the clusiaceae Hypericum plantdifferent extract parts of anti-tachyarrhythmia role and the material basis. Through research the affection of the separation of the monomer substances on the myocardial membrane Ca+-Mg2+-ATP enzymes and Na+-K+-ATP enzymes, analyzing the possible mechanism against tachyarrhythmia role of the Hypericum attenuatum Choisy.Methods:1. With the Medlab bio-signal acquisition and processing system,It is on the basis of the automatic samplingcomputer analysis of ECG recording that considering the effect of the Hypericum attenuatum Choisy extract towardsto experimental arrhythmias which caused by the two methods of aconitineand calcium chloride.2. According to the Langendorff technique, chromatography, and spectroscopic identification method of modern science and technology, we isolated and identified two kinds of compounds from the effective parts of the Hypericum attenuatum Choisy antiarrhythmic.3. Make use of the inorganic phosphorus colorimetric assay to detect the Hypericum attenuatum Choisy antiarrhythmic so that isolation and identification two kinds of compounds on rat myocardial membrane Ca+-Mg+-ATP enzymes and Na+-K+-ATP enzymes.Result:1. It is so clear that butanol extraction group of Hypericum attenuatum Choisy were able to extend the time of appearance of the acomitine, calcium chloride induced rat tachyarrhythmia, shorten the arrhythmia duration. In addition, It has a statistically significant difference compared with model group.(P<0.05). Therefrom, butanol group was superior to the ethyl acetate extract group. And the butanol extraction group was better than the ethyl acetate extract group.2. We repeated separation and pharmacological experiments butanol from the Hypericum attenuatum Choisy extracts and the two kinds of compounds were separated and identified which are hypericin and hyperoside.3. The hyperin can improve tachyarrhythmia rat cardiac membrane Na+-K+-ATP enzymes and Ca2+-Mg2+-ATP enzymes activity which compared with the blank group,the difference was statistically significant.(P<0.05)Conclusion:1. It has a significant antagonism to the tachyarrhythmia animal models induced by aconitine and calcium which shows that Hypericum attenuatum Choisy has a good job on it.2. Rutin and Hyperoside were the active ingredient to anti-tachyarrhythmia.The former is better which is he basis material to anti-tachyarrhythmia.3. Hyperoside that effective compounds isolated from the Hypericum attenuatum Choisy can improve tachyarrhythmia rat cardiac membrane Na+-K+-ATP enzymes and Ca2+-Mg2+-ATP enzymes activity. It is show that the anti-tachyarrhythmia mechanism may regulate with the myocardial membrane Na+-K+-ATP enzymes and Ca2+-Mg2+-ATP enzymes activity. The rutin don’t have such pharmacological effects, It may through the cooperation function to the anti-tachyarrhythmia.更多还原