【作者】 王煜；

【导师】 孙连坤；

【作者基本信息】 吉林大学 ， 病理学与病理生理学， 2010， 硕士

【摘要】 目的:通过免疫组织化学方法检测人肾细胞癌中Notch信号途径蛋白的表达水平,探究Notch信号途径分子与人肾细胞癌发生发展的可能关系,为临床寻找新的治疗肾细胞癌的靶点提供一定的方向和理论依据。方法:手术方法获得患者的肾细胞癌组织及癌旁组织标本,共8对,通过HE染色和免疫组织化学染色的方法对肾细胞组织和癌旁组织中Notch信号通路受体Notch-1、Notch-2和配体Jagged-1、Jagged-2蛋白表达水平进行检测。结果:癌症组织中Notch信号途径蛋白Notch-1、Jagged-1、Notch-2以及Jagged-2阳性率分别为22.5%、30%、65%和60%;癌旁组织中Notch信号途径蛋白Notch-1、Jagged-1、Notch-2以及Jagged-2阳性率分别为72.5%、67.5%、27.5%和20%,癌旁组织与癌症组织Notch-1、Jagged-1、Notch-2以及Jagged-2阳性率比较差异有统计学意义(P﹤0.05)。结论:1. Notch信号通路蛋白和人肾细胞癌的发生呈现出一定的关联性。2. Notch信号通路的受体Notch-1和配体Jagged-1在人肾细胞癌组织中的表达阳性率明显低于癌旁组织,提示Notch-1和Jagged-1与肾细胞癌的发生呈现出了负相关。3. Notch信号通路的受体Notch-2和配体Jagged-2在人肾细胞癌组织中的表达阳性率明显高于癌旁组织,提示Notch-2和Jagged-2与肾细胞癌的发生呈现出了正相关。更多还原

【Abstract】 Objective:Detecting the Notch signaling pathway related protein expression level by immunohistochemistry in human renal cell carcinoma,to explore the possible relationship between the Notch signaling pathway moleculars and the development of human renal cell cancer,providing some direction and theoretical basis for finding new targets in the clinical treatment of renal cell carcinoma.Method:Obtain of 8 pieces the renal cell carcinoma specimens by surgical methods, the Notch signaling receptor Notch-1, Notch-2 and ligand Jagged-1, Jagged-2 protein levels in renal cell tissue and renal cell carcinoma adjacent tissues were detected by HE and immunohistochemical staining methods.The results showed that there are some relevance of the occurrence of renal cell carcinoma and Notch signaling pathways, and that the Notch signaling pathway may be involved in the development of renal cell carcinoma. Results:The positive rate of the memberof Notch signaling pathway Notch-1, Jagged-1,Notch-2 and Jagged-2 was 22.5% , 30%, 65% and 60% , in renal cell carcinoma ,respectively. The positive rate of the member of Notch signaling pathway Notch-1, Jagged-1,Notch-2 and Jagged-2 was 72.5% , 67.5%, 27.5% and 20% , in renal cell carcinoma adjacent tissue,respectively. Compared with renal cell carcinoma adjacent tissue, the positive rate of the memberof Notch signaling pathway Notch-1, Jagged-1,Notch-2 and Jagged-2 have statistical significance in renal cell carcinoma(p﹤0.05).Conclusion:1. It showed that there are some relevance of the Notch signaling pathway protein and the development of human renal cell carcinoma .2. Compared with the renal cell carcinoma adjacent tissue, the expression levels of the Notch signaling and Notch-1 receptor ligand Jagged-1 in the cancer tissue were significantly lower, suggesting that there is a negative relevance of Notch-1 and Jagged-1 with the development of renal cell carcinoma.3. Compared with the renal cell carcinoma adjacent tissue, the expression levels of Notch-2, another receptor of Notch signaling pathway, and its ligand Jagged-2 in the cancer tissue were significantly higher, suggesting that there is a positive relevance of Notch-1 and Jagged-1 with the development of renal cell carcinoma.更多还原