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新疆传统发酵乳酪乳清中发酵菌的分离鉴定
Isolation and Identification of Zymocyte from the Traditional Fermentation of Cheese Whey (TFCW) in Xinjiang
【作者】 马春燕；
【导师】 新华·那比；
【作者基本信息】 新疆医科大学 ， 药理学， 2010， 硕士
【摘要】 目的:在前期的研究中已证实新疆传统发酵乳酪乳清(简称乳酪乳清,TFCW)具有调血脂、抗炎、抗氧化和抗动脉粥样硬化作用,推断该功能可能与发酵菌及其产生的活性代谢产物(如生物活性肽等)有关。基于以上成果,本研究对TFCW中发酵菌进行分离鉴定,分析该菌种在乳酪乳清抗动脉粥样硬化中的作用机制。方法:采集新疆阿勒泰牧区传统发酵乳酪乳清,通过多种选择性培养基对其进行分离培养,结合传统鉴定方法(形态学观察和生化特性鉴定)和基因序列分析方法进行鉴定。结果:本研究发现TFCW中所含的发酵菌主要为乳酸菌和酵母菌。(1)乳酸菌为7株、分属于2个属,即乳杆菌属和乳球菌属。乳杆菌属又包括6个种,分别为短乳杆菌( Lactobacillus brevis )、植物乳杆菌(Lactobacillus plantarum)、瑞士乳杆菌(Lactobacillus.helveticus)马乳酒样乳杆菌(Lactobacillus kefianofaciens)、干酪乳杆菌(Lactobacillus casei)和香肠乳杆菌(Lactobacillus farciminis)。乳球菌属包括1个种:乳酸乳球菌(Lactococcus lactic);(2)酵母菌为2株,分属于2个属:单孢酿酒酵母(Saccharomyces unisporus)和东方伊萨酵母(Issatchenkia orientalis)。它们分属于酵母属中和伊萨酵母属。结论:TFCW抗动脉粥样硬化及其抗氧化等作用可能与所含发酵菌种类及其该发酵菌产生的活性代谢物质(ACE抑制肽及抗氧化肽等)有关。
【Abstract】 Objective: TFCW has been confirmed with anti-inflammatory,anti-oxidant and anti- atherosclerosis effects in the pre-research of our group, it is speculated that the result may be related to the active metabolites of zymocyte, such as biologically active peptide.Therefore,zymocyte was isolated and identificated.This will provide a basis for the research of mechanism of action of the active metabolites of zymocyte.Methods:TFCW samples were collected from Altay in XinJiang.Zymocyte was isolated from TFCW by using selective media,then they were identified in traditional method and gene sequencing analysis.The traditional method contain morphological and biochemical approach. Results: The result showed that the selected zymocyte stains from TFCW belonged to lactic acid bacteria and yeast.Lactic acid bacteria includ 6 strains of Lactobacillus,.respectively:Lactobacillus brevis,Lactobacillus plantarum,Lactobacillus.helveticus,Lactobacillus kefianofaciens, Lac- tobacillus casei,Lactobacillus farciminis and 1brains of Lactococcus which is Lactococcus lactic.Yeast includ 2 strains,.respectively: Saccharomyces unisporus and Issatchenkia orientalis,and they belonged to Saccharomyces and Issatchenkia.Conclusion:The anti- atherosclerosis and anti-oxidant effects of TFCW confirmed in our pre-experiments are related to zymocyte species and the active metabolites of zymocyte. active metabolites may be ACE inhibitory peptides and anti-oxidant peptides and so on..