【作者】 周静；

【导师】 陈国强；

【作者基本信息】 汕头大学 ， 生物化学与分子生物学， 2010， 硕士

【摘要】 睑板修复是眼睑重建的重要部分。目前的传统治疗手段并不能很好地满足修复需要。因此寻找一种合适的人工睑板替代物成为关键问题。本实验中研究了产自微生物的3-羟基丁酸3-羟基己酸共聚物(PHBHHx)作为人工睑板替代材料的应用潜能。将PHBHHx支架置入Sprague-Dawley大鼠的睑板缺损部位,并在术后第1、2、4、8周分别收集实验组、对照组和空白组大鼠的术眼眼睑进行组织学检测鉴定。对PHBHHx支架和ADM的植入效果以及空白组的术后情况进行比较。结果表明,尽管PHBHHx材料植入后的最初两周内呈现出较明显的炎症反应,但较之空白组,实验组的PHBHHx支架和对照组的ADM材料均表现出较好的修复效果。此外,在实验组中还可以观察到PHBHHx植入部位出现了胶原包裹;同时,支架也出现了降解现象。由于PHBHHx材料综合了由软至刚的可控性能,又具有组织相容性和生物可降解性,因此可能成为人工睑板修复材料的较合适选择。更多还原

【Abstract】 Tarsal repair is an important part for eyelid reconstruction. Presently traditional clinic treatments do not produce satisfactory repair effects. The key is to find a proper artificial tarsal repair material. Microbial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) was studied for application as artificial tarsal substitute in this study. PHBHHx scaffolds were implanted into tarsal defects of Sprague-Dawley rats. Eyelid samples of implanted materials and blank defect controls were collected for histological examination at weekly intervals post surgery. Results were compared among PHBHHx scaffolds, commercial acellular dermal matrices (ADM) and blank defect controls. Both PHBHHx scaffolds and ADM provided satisfactory repair results compared with the blank controls even though the implanted PHBHHx scaffolds showed a two weeks inflammation. Fibrous encapsulation and scaffold degradation were observed for the PHBHHx implants. Combined with its strong, soft mechanical properties, the tissue compatible and biodegradable PHBHHx was proven to be a suitable candidate for artificial tarsal repair.更多还原