反复呼吸道感染患儿中医体质证型与免疫功能的关系探讨

【作者】 孟祥萍；

【导师】 宋铁玎；

【作者基本信息】 辽宁中医药大学 ， 中医儿科学， 2010， 硕士

【摘要】 目的:对反复呼吸道感染(RRTI)患儿进行中医体质分型,并对其进行外周血T淋巴细胞亚群和免疫球蛋白测定,探讨RRTI患儿中医体质证型与T淋巴细胞亚群、免疫球蛋白的关系。研究方法:对47例复感儿进行中医体质分型,并进行外周血T淋巴细胞亚群( CD3~+、CD4~+、CD8~+、CD4~+/CD8~+)和免疫球蛋白(IgA、IgG、IgM)检测,所得数据运用SPSS15.0 for windows统计软件包进行处理分析; T淋巴细胞亚群采用美国BD公司生产的BD FacsCalibur流式细胞仪,静脉血2ML(抗凝);免疫球蛋白(静脉血清2ML)采用日立760全自动生化分析仪(本实验均由本院检验中心完成)。结果1.47例复感儿的中医体质证型分布不均,以气虚质(42.6%)、阴虚质(40.4%)为多,其他体质证型较为少见。2.两个研究组CD4~+、CD4~+/CD8~+、IgA、IgG较对照组均降低,按照气虚质-阴虚质的顺序逐渐降低,差异均有统计学意义(P<0.05) CD8~+均升高,差异无统计学意义(P>0.05)。3.气虚质组较阴虚质组CD4~+降低,差异有统计学意义(P<0.05)。结论1.47例复感儿的中医体质证型分布不均,以气虚质(42.6%)、阴虚质(40.4%)为多。2.气虚质和阴虚质复感儿T淋巴细胞亚群和免疫球蛋白与正常质组比较均有一定程度的紊乱或降低。3.T淋巴细胞亚群CD3~+、CD4~+、CD8~+和免疫球蛋白(IgA, IgG, IgM)能否作为体质分型的客观指标尚有待于进一步研究。更多还原

【Abstract】 Objective: Preschool recurrent respiratory tract infection (RRTI) for Chinese children with constitution type, and their peripheral blood T lymphocyte subsets and immunoglobulin, the type of TCM constitution RRTI children and T lymphocyte subsets Immunoglobulin.Methods: 47 patients with complex sense of children of Chinese medicine practitioners constitution type, and peripheral blood T lymphocyte subsets (CD3 ~+, CD4 ~+, CD8 ~+, CD4 ~+ / CD8 ~+) and immunoglobulin (IgA, IgG, IgM) detection, the data SPSS15.0 for windows statistical package used to process analysis; T cell subsets produced by the U.S. BD BD FacsCalibur flow cytometry, blood 2ML (anticoagulant); immune globulin (intravenous serum 2ML) using Hitachi 760 automatic biochemical analyzer (in this experiment completed by the hospital test center).Result1.47 cases of Chinese children with complex constitution type of a sense of uneven distribution, with Qi Deficiency quality (42.6%), yin deficiency (40.4%)were more than other constitution types of more rare.2. The two study groups CD4 ~+, CD4~+ / CD8 ~+, IgA, IgG were lower than the control group, according to Qi deficiency of quality - yin deficiency the order decreased, the differences were statistically significant (P <0.05) CD8~+ are high, the difference no statistically significant (P> 0.05).3. Qi deficiency quality group lower than yin deficiency group in CD4 ~+ the difference was statistically significant (P <0.05).Conclusion1.47 cases of Chinese children with complex constitution type of a sense of uneven distribution, with Qi Deficiency quality (42.6%), yin deficiency (40.4%) were more. 2. Qi Deficiency quality and Yin Deficiency quality of children T lymphocyte subsets and immunoglobulin compared with the normal quality there is a certain degree of disorder or lower.3.T lymphocyte subsets CD3 ~+, CD4~+, CD8~+, and immunoglobulin (IgA, IgG, IgM) whether as an objective index of the constitution is to be in a further study.更多还原