【作者】 秦积龙；

【导师】 古力娜尔·库尔班；

【作者基本信息】 新疆医科大学 ， 病理学与病理生理学， 2010， 硕士

【摘要】 目的探讨脆性组氨酸三联体（fragile histidine triad ,FHIT）、p16^INK4a与视黄酸受体β（retinoic acid receptor-beta,RARB）蛋白在新疆维吾尔族妇女宫颈上皮内瘤变（cervical intra-epithelial neoplasia,CIN）及宫颈癌中的表达及其意义。方法采用免疫组化链酶卵白素-辣根过氧化物酶（SP）法检测20例慢性宫颈炎、30例CIN（CINⅠ10例、CINⅡ10例、CINⅢ10例）以及40例浸润性宫颈鳞癌组织标本中FHIT、p16^INK4a及RARB蛋白的表达。结果（1）FHIT蛋白阳性表达率依次为慢性宫颈炎（90.00%）>CIN（66.67%）>浸润性宫颈鳞癌（27.50%）,差异具有统计学意义（P=0.000）;p16^INK4a蛋白阳性表达率依次为浸润性宫颈鳞癌（82.50%）>CIN（46.67%）>慢性宫颈炎（0.00%）,差异都具有统计学意义（P=0.000）;RARB蛋白阳性表达率依次为浸润性宫颈鳞癌（90.00%）>CIN（53.33%）>慢性宫颈炎（10.00%）,差异都具有统计学意义（P=0.000）。（2）在宫颈各组织中,FHIT蛋白与p16^INK4a蛋白表达呈负相关（r =-0.384,P=0.000）;FHIT蛋白与RARB蛋白表达呈负相关（r =-0.291,P=0.006）;p16^INK4a蛋白与RARB蛋白表达呈正相关（r = 0.445,P =0.000）。结论抑癌基因FHIT表达缺失与p16^INK4a和RARB过度表达与宫颈癌的发生发展密切相关,并且FHIT、p16^INK4a、RARB具有协同作用。FHIT蛋白与p16^INK4a蛋白和RARB蛋白的联合检测可作为宫颈癌早期诊断和宫颈癌进展的分子指标。更多还原

【Abstract】 Objective To stdudy expression and clinical significance of the fragile histidine triad （fragile histidine triad, FHIT）, p16^INK4a and retinoic acid receptorβ（retinoic acid receptor-beta, RARB） protein in the Xinjiang Uygur women cervical intraepithelial neoplasia （cervical intra-epithelial neoplasia , CIN） and cervical cancer. Methods Using immunohistochemical streptomycin avidin-peroxidase link （SP） method to detect expression of FHIT, p16^INK4a and RARB protein of 20 cases of chronic cervicitis, 30 cases of CIN （CINⅠ10, CINⅡ10, CINⅢ10） and 40 cases of invasive cervical squamous cell carcinoma specimens. Results （1） FHIT protein expression was followed by chronic cervicitis （90.00%） > CIN （66.67%） > invasive cervical squamous cell carcinoma （27.50%） and the difference was statistically significant （P = 0.000）;Expression of p16^INK4a and RARB protein was followed by invasive cervical squamous cell carcinoma （82.50%; 90.00%） > CIN （46.67%; 53.33%） > chronic cervicitis （0.00%; 10.00%） and the difference is statistically significant （P = 0.000; P = 0.000）. （2） In Various cervix uteritissue, the expression of FHIT and p16^INK4a protein was negatively correlated （r = -0.384, P = 0.000）; The expression of FHIT and RARB was negatively correlated （r = -0.291, P = 0.006）; The expression of p16^INK4a and RARB protein was positively correlated （r = 0.445, P = 0.000）. Conclusion Expression and deletion of tumor suppressor gene - FHIT and overexpression p16^INK4a and RARB is closely related to the genesis and development of cervical cancer. The protein of FHIT, p16^INK4a and RARB have a synergistic effect in cervical cancer. The detection FHIT, p16^INK4a and RARB protein can be considered as a early detection index of cervical cancer and progress of cervical cancer.更多还原