【作者】 王成磊；

【导师】 张佩青；

【作者基本信息】 黑龙江省中医研究院 ， 中医内科学， 2010， 硕士

【摘要】 目的:探讨肾炎消白颗粒减少阿霉素肾病大鼠蛋白尿的作用,并从生化、分子生物学角度探讨其作用机理,为临床应用提供理论依据。方法:60只Wistar大鼠,随机分为5组,分别为空白对照组、模型对照组、洛汀新组、肾炎消白颗粒低剂量组、肾炎消白颗粒高剂量组,用尾静脉一次性注射阿霉素制备肾炎蛋白尿模型,而后各组以预定方法灌胃,观察7周,于灌胃前一天及以后每七天检测大鼠的尿肌酐,酶联免疫法检测尿白蛋白,第七周末ELISA法检测尿β2-MG,免疫组织化学法检测大鼠肾组织中Nephrin蛋白表达情况。结果:实验7周末各治疗组(肾炎消白颗粒高、低剂量组及洛汀新组)阿霉素肾病大鼠尿白蛋白/尿肌酐值下降,以肾炎消白颗粒高剂量组最为明显(P<0.05);尿β2-MG的含量降低,以肾炎消白颗粒高剂量组最为明显(P<O.05);Nephrin在肾组织中的表达增加,以肾炎消白颗粒高剂量组最为明显(P<0.05)。结论:肾炎消白颗粒能够在一定程度上减少阿霉素肾病大鼠尿蛋白的排泄量;减少阿霉素肾病大鼠尿β2-MG的含量,对肾小管有保护作用;增加阿霉素肾病大鼠Nephrin在肾组织中的表达,对肾脏纤维化有治疗作用;中药高剂量组对于上述作用较中药低剂量组、洛汀新组疗效较好。更多还原

【Abstract】 Objective:To test if nephritis Xiaobaikeli decreases proteinuria level in experimental animals and investigate its mechanism by biochemical and molecular biological analysis to provide the basis for its clinical application.Methods:We utilized a rat model in which nephritis is induced by one-time tail vein injection of doxorubicin.Animals were divided into five groups,namely the naive control group,model control group,Lotensin group,low-dose nephritis Xiaobaikeli group and high-dose nephritis Xiaobaikeli group.Seven weeks after treatment,we detected rat urine creatinine,urine Scr,urineβ2-MG(by ELISA),proteinuria,Nephrin expression in kidney(by immunohistochemical staining),.Results:At the end of 7-week observation,the ratio of proteinuria/urine creatinine values(P<0.05)were reduced in animals from all treatment group with doxorubicin-induced nephritis,and the high-dose nephritis Xiaobaikeli treatment had the most significant effects(P<O.05.);the urineβ2-MG level(P<O.05)was also decreased in all treatment group,and the high-dose nephritis Xiaobaikeli treatment had the most significant effects (P<O.05); the expression of Nephrin in the kidney were increased in all treatment group(P<0.05).and the high-dose nephritis Xiaobaikeli treatment had the most significant effects (P<0.05).Conclusion:nephritis Xiaobaikeli can reduce excretion a certain extent,decrease urineβ2-MG level in nephrotic rats and protect tubulo: It can increase Nephrin expression in kidney tissues. The therapeutic effects with high-dose nephritis Xiaobaikeli are better than low-dose nephritis Xiaobaikeli or Lotensin.更多还原