不同产地蜂胶的质量评价研究

【作者】 王维；

【导师】 邱智东；

【作者基本信息】 长春中医药大学 ， 中药学， 2010， 硕士

【摘要】 蜂胶为蜜蜂科昆虫意大利蜂Apis mellifera L.的干燥分泌物[l]。现代药理学研究表明蜂胶具有抗菌消炎、调节免疫、抗氧化、加速组织愈合等作用。临床上用于高脂血症和糖尿病的辅助治疗,目前蜂胶已广泛地应用于医药、保健品、化妆品等领域。本文首先对不同产地蜂胶的质量的进行了初步评价。根据《中国药典》2005版收载的蜂胶项下的规定,对13个不同产地的蜂胶分别进行了薄层鉴别以及干燥失重、总灰分、酸不溶性灰分、氧化时间、醇浸出物检查。采用HPLC法测定了13个产地蜂胶中的白杨素及高良姜素的含量,同时采用紫外分光光度法测定了蜂胶总黄酮的含量。结果表明,吉林省蛟河及河南省长葛地区的蜂胶品质较优,同时提示,不同产地的蜂胶质量存在较大差异。本文采用HPLC-DAD法对蜂胶进行指纹图谱研究,采用Agilent Zorbax SB-C18 (250mm×4.6mm, 5μm)色谱柱,以甲醇-0.1%甲酸为流动相梯度洗脱,检测波长为290nm;流速为0.8ml/min,得到分离度较好的蜂胶指纹图谱,以期为蜂胶全面质量控制提供参考。采用液质联用技术对蜂胶指纹图谱进行了定性分析,推测了其中18个峰的可能结构,其中9个黄酮类化合物(2个为黄酮类化合物和7个为二氢黄酮类化合物),9个芳香族酸及芳香族酸的酯类化合物(3个为芳香族酸类化合物和6个为芳香族酸的酯类化合物),为蜂胶的药效物质成分提供更多的化学信息,以期为今后能够更加全面、合理的评价蜂胶的质量提供更多的科学依据。更多还原

【Abstract】 Propolis is dry secretions from the bee Apis mellifera L.of Italian insects.Modern pharmacological studies have shown that propolis has anti-bacterial anti-inflammatory, regulating immunity, anti-oxidation, accelerate tissue healing and so on. Propolis is used adjuvant therapy for hyperlipidemia and diabetes in the clinic. The present propolis has been widely used in medicine, health products, cosmetics and other fields.In this paper, I have conducted the preliminary evaluation based on the quality of different sources of propolis. According to the 2005 edition of China pharmacopoeia under the provisions of propolis against 13 different origin of propolis were carried out by TLC, as well as loss on drying, total ash, acid insoluble ash, oxidation time, alcohol extract inspection. It were determined using HPLC method in 13 propolis origin chrysin and galangin content, At the same time were determined by ultraviolet spectrophotometry of flavonoids in propolis. The results show that, the propolis in Jilin Province Jiaohe and Henan Province Changge areas are superior to others areas.In this paper, using HPLC-DAD method on fingerprint of propolis research, using Agilent Zorbax SB-C18 (250mm×4.6mm, 5μm) column with methanol -0.1% formic acid as mobile phase gradient elution; detection wavelength 290nm; velocity To 0.8ml/min,we obtain a better separation of propolis fingerprints with a view to provide reference for total quality control of propolis.Using LC-MS techniques for propolis fingerprints to qualitativly analyze, speculating the possible structure of 18 peaks, of which 9 flavonoid compounds (2 for the flavonoids and 7 for the dihydro-flavonoids), 9 aromatic acid and aromatic acid esters (3 for the aromatic acids and 6 for the aromatic acid esters), providing more chemical information for the efficacy material composition of propolis, with a view to the future will be more comprehensive and rational evaluation of the quality of propolis to provide more scientific basis.更多还原