【作者】 苏桃；

【导师】 陈临溪；

【作者基本信息】 南华大学 ， 药理学， 2010， 硕士

【摘要】 目的:研究G蛋白偶联受体APJ及其内源性配体apelin是否与肺癌的发生发展有关,是否参与肺癌细胞的生长增殖,发现apelin/APJ的新生物学功能,为临床诊断和治疗肺癌以及抗癌药物的开发提供新的思路.方法:1.免疫组织化学法:观察APJ在人肿瘤组织中的表达;2. ELISA:检测肿瘤病人血浆apelin的含量;3.免疫荧光法:检测人肺腺癌细胞系A549中APJ定位;4. Western Blot:检测人肺腺癌细胞系A549、胃腺癌细胞系BGC823、肝癌组织中APJ的表达;5.细胞增殖检测法:CCK-8试剂盒检测apelin-13对肺腺癌细胞系A549增殖的影响.结果:1. APJ在肺腺癌、肺鳞癌、子宫肌瘤、肝细胞癌、胃腺癌、宫颈癌、星形细胞瘤的肿瘤细胞中表达较相应癌旁组织明显增加;2.肺癌、宫颈癌、胃癌病人血浆中apelin的含量高于对照组;3. APJ在肺腺癌细胞系A549中表达,主要分布于细胞膜与细胞质;4. APJ在人肺腺癌细胞、胃腺癌细胞、肝癌组织中均有表达,APJ在肺腺癌细胞中表达高于胃腺癌细胞与肝癌组织;5. Apelin-13能促进肺腺癌细胞系A549增殖,呈时间、浓度依赖关系,其中浓度为1μmol/L、时间为24h作用最明显.结论:1.人肺腺癌、肺鳞癌、子宫肌瘤、肝细胞癌、胃腺癌、宫颈癌、星形细胞瘤APJ受体表达明显增加;2. Apelin/APJ系统通过促进肺癌细胞增殖参与肺癌的发生发展.更多还原

【Abstract】 Aim:1. To study the relationship between apelin/APJ system and human lung cancer;2. To observe the effect of apelin on the proliferation of human lung adenocarcinoma cell line A549.Methods:1. The distribution of APJ in human tumor tissues was detected by Immunohistochemistry staining;2. The plasma apelin level in tumor patients was detected by ELISA;3. The distribution of APJ in human lung adenocarcinoma cell line A549 was detected by Immunofluorescence;4. The expression of APJ in human lung adenocarcinoma cell line A549, human gastric adenocarcinoma cell line BGC823 and liver cancer tiusse was detected by Western blot;5. The effect of apelin-13 on the proliferation human lung adenocarcinoma cell line A549 was detected by CCK-8 assay.Results:1. The expression of APJ in cancer cells of lung adenocarcinoma,lung squamous cell carcinoma,hysteromyoma,liver cancer,gastric cancer,cervical carcinoma was higher than noncancerous tissues;2. The plasma apelin level in lung cancer patients,cervical cancer patients and gastric cancer patients was higher than normal persons;3. The APJ expressed on the membrane and cytoplasma of human adenocarcinoma cell line A549;4. The APJ expressed in lung adenocarcinoma cell line A549, gastric adenocarcinoma cell line BCG823 and liver cancer tissue. The expression of APJ in A549 was higer than BGC823 and liver cancer tissue; 5. Apelin-13 promoted the proliferation of A549 cell in concentration and time dependent manner.Conclusion:The expression of APJ in cancer cells of lung adenocarcinoma, lung squamous cell carcinoma, hysteromyoma, liver cancer, gastric cancer, cervical carcinoma was higher than noncancerous tissues. Apelin could promote the proliferation of human lung adenocarcinoma cell line A549. Apelin/APJ was probably involved in the pathogenesis of human lung cancer and other tumors.更多还原