【作者】 王雷；

【导师】 尹增芳；

【作者基本信息】 南京林业大学 ， 植物学， 2010， 硕士

【摘要】 本论文以南林895扦插苗为试验材料,采用形态指标检测、生理生化测定、稳定同位素分析等技术,研究了外源Ca²⁺处理下南林895杨扦插苗的营养生长状况,探讨了外源Ca²⁺对南林895杨扦插苗光合同化物分配格局的响应,结果表明:1.外源Ca²⁺能够促进南林895杨扦插苗的营养生长。喷施外源Ca²⁺后,扦插苗的株高、地径、生物量均优于对照,其中以200mg/L浓度水平的Ca²⁺处理效果最好;2、20mg/L Ca²⁺对扦插苗的促进效应不明显,300mg/L Ca²⁺浓度对扦插苗的促进效应相对较弱。2.外源Ca²⁺可以改善南林895杨扦插苗光合作用效率。不同浓度Ca²⁺处理下扦插苗净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）、细胞间CO2浓度（Ci）均呈现先升高后下降的趋势。随着Ca²⁺浓度的升高,20mg/L Ca²⁺处理下Ci值最大;但Pn、Tr、Gs在200mg/L Ca²⁺处理下才达到最大值,与对照差异性显著。3.外源Ca²⁺可以提高南林895杨扦插苗叶绿素、可溶性蛋白质的含量。较高浓度的Ca²⁺（100～300mg/L）对植株的影响作用较大,在处理20d前,以200mg/L Ca²⁺处理效果最佳;25d后以100mg/L Ca²⁺处理效果最好。4.外源Ca²⁺可以调控南林895杨扦插苗可溶性糖含量及其糖代谢相关酶活性的动态变化。“源”器官可溶性糖含量及代谢相关酶活性高于“库”器官,推测外源Ca²⁺提高了南林895杨扦插苗“库”（茎）的强度,促进其光合同化物由“源”（叶）向“库”中转运。5.外源Ca²⁺影响南林895杨扦插苗营养器官对稳定同位素碳、氮的富集能力。随着Ca²⁺浓度的升高,δ13C、δ15N值均呈现逐渐上升然后缓慢下降的趋势。δ13C、δ15N值在植株中的分布遵从“库”器官大于“源”器官的规律。6.外源Ca²⁺对南林895杨扦插苗生长的促进作用具有一定的累积效应。低浓度Ca²⁺随着时间的延长而作用效果加强,而高浓度Ca²⁺随着生长期的延长其作用效果逐渐减弱。更多还原

【Abstract】 Using the methods of morphological index measuring, physiological and biochemical testing, stable isotope analysis techniques, we investigated the dynamic changes of regularity on vegetative growth status of Populus×euramericana‘Nanlin 895’cuttings under different concentration of exogenous Ca²⁺ treatment. And that we also explored the effects of exogenous Ca²⁺ on photo-assimilate allocation patterns of Populus×euramericana‘Nanlin 895’cuttings. The results showed as follows:1. The exogenous Ca²⁺was able to accelerate the vegetative growth of Populus×Eurameri cana‘Nanlin 895’cuttings. The plant height, caliper, bio-yield were superior to control test after spraying different concentration of exogenous Ca²⁺. Thereinto, the 200mg/L Ca²⁺concentration treatment was the best one especially. The 2, 20mg/L Ca²⁺concentrations treatment had a slightly positive effect which was compared with the control test. And the enhance effect would be decreased if the Ca²⁺ concentration reached to 300mg/L.2. The exogenous Ca²⁺could promote the photosynthesis efficiency of Populus×eurameri cana‘Nanlin 895’cuttings. With the Ca²⁺ concentration increasing, the Pn、Tr、Gs、Ci of leaves were presented gradually trends of increase in advance, and then decrease without exception. The value of Ci would reach to maximum when the Ca²⁺ concentration treatment was 20mg/L. However, the value of Pn、Tr、Gs would reach to the peak when the concentration exogenous Ca²⁺ concentration treatment was 200mg/L.3. The exogenous Ca²⁺ could increase the chlorophyll and soluble protein content of Populus×euramericana‘Nanlin 895’cuttings. And that the relative higher Ca²⁺ concentration presented an obvious stimulative effect. The 200mg/L Ca²⁺ was the optimal concentration before spraying 20 days, while the 100mg/L Ca²⁺ was the optimal concentration after 25 days.4. The exogenous Ca²⁺ could regulate the dynamic changes of soluble sugar content and sugar-metabolizing related-enzyme of Populus×euramericana‘Nanlin 895’cuttings. The soluble sugar content and the related enzyme activity of "sink" organ were higher than "source" organ. It was speculated that the exogenous Ca²⁺ could enhance the "sink’s" strength, and accelerate the assimilation transportation from "source".5. The stable isotope value of carbon and nitrogen enrichment capacity of Populus×euramericana‘Nanlin 895’cuttings were affected by exogenous Ca²⁺. With the Ca²⁺concentration increasing, theδ13C,δ15N value of cuttings were presented gradually trends of increase in advance, and then decrease without exception. The distribution of plants’δ13C,δ15N value should be obeyed the principle that the "sink" organ was bigger than the "source" organ.6. It had a certain accumulative effect that the exogenous Ca²⁺ played a vital role in promoting the growth of Populus×euramericana‘Nanlin 895’cuttings. The positive effect of lower Ca²⁺ concentration gradually were enhanced, while the negative effect of higher Ca²⁺ concentration has gradually started to emerge as the extension of growth period.更多还原